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摘要: 目的:对8例ABO血型B亚型进行血清学检测并分析,为临床鉴定正反定型不符疑难血型提供借鉴。方法:采用微柱凝胶卡式法及试管法进行ABO血型定型,并应用红细胞吸收放散试验、唾液中HAB血型物质测定等鉴定亚型。结果:8例患者样本红细胞上均存在B抗原,并经血清学鉴定均符合Bx亚型分类标准。结论:Bx亚型由于红细胞所含B抗原极弱,易被误定为O型,在亚型红细胞出现正反定型不符的情况下,应使用包括血清学在内的多种方法正确鉴定其血型,以保证输血安全。Abstract: Objective:To serological detect and analyze 8 samples of ABO blood group of B subtype,and provide the reference for clinical identification of reciprocal stereotypy about mismatching difficult blood type.Method:ABO blood group was detected using micro-column gel card test and tube test,and subtype was identified by absorption elution test of red blood cell and matter detection of saliva's HAB blood group.Result:The 8 samples of red blood cell existed antigen-B,which matched Bx subtype categorization standard through serology identification.Conclusion:Bx subtype was identified to be O type easily,because of significantly weak antigen-B on its red blood cell.Under the circumstance of subtype red blood cell showing ABO typing discrepancy,all kinds of methods including serology detection should be used to identify the blood group correctly for ensuing the security of transfusion.
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Key words:
- ABO blood group /
- Bx subtype /
- serology
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