Effect of GRP78 in macrophage polarization
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摘要: 目的：探讨葡萄糖调节蛋白78（GRP78）对单核巨噬细胞分化的影响。方法：利用佛波酯（PMA）诱导形成THP-1单核细胞来源巨噬细胞模型。实验分为4组，分别为对照组（PMA浓度为50 ng/ml）、GRP78 10 ng/ml组（GRP78浓度10 ng/ml+PMA 50 ng/ml）、GRP78 20 ng/ml组（GRP78浓度20 ng/ml+PMA 50 ng/ml）、GRP78 40 ng/ml组（GRP78浓度40 ng/ml+PMA 50 ng/ml），培养72 h。应用流式细胞术检测巨噬细胞M1型特异表面标志物CD86、M2型特异表面标志物CD163的表达；实时荧光定量PCR检测巨噬细胞M1型相关因子iNOS、M2型相关因子FIZZ-1、TGF-β、ARG-1及IL-10的mRNA表达。结果：与对照组比较，其他3组的CD163阳性巨噬细胞百分比显著增加（P<0.01）；而各实验组CD86阳性巨噬细胞百分比没有明显改变。与对照组相比，GRP78实验组中各组FIZZ-1、TGF-β的mRNA水平均有增加，其中GRP78 40 ng/ml组FIZZ-1、TGF-β的mRNA水平明显增高（P<0.01）；各组其他因子iNOS、IL-10和ARG-1的mRNA水平则没有明显变化。结论：GRP78可诱导THP-1单核巨噬细胞极化成M2型巨噬细胞。Abstract: Objective: To study the influence of glucose-regulated protein 78(GRP78) on the macrophage polarization.Method: THP-1 derived macrophages model differentiated by phorbol 12-myristate 13-acetate(PMA) was established.The experiment was divided into four groups:control group(50 ng/ml PMA concentration), GRP78 10 ng/ml group(GRP78 10 ng/ml + PMA 50 ng/ml), GRP78 20 ng/ml group(GRP78 20 ng/ml + PMA 50 ng/ml), and GRP78 40 ng/ml group(GRP78 40 ng/ml + PMA 50 ng/ml).All groups were cultured for 72 h.The expressions of M1 surface marker CD86 and M2 surface marker CD163 were detected by flow cytometry.The expressions of the M1 polarization related-gene(iNOS) and M2 polarization related-genes(FIZZ-1, TGF-β, ARG-1 and IL-10) were analyzed by qPCR.Result: Compared with the control group, the percentages of CD163 positive macrophages in GRP78 10 ng/ml group, GRP78 20 ng/ml group and GRP78 40 ng/ml group were increased significantly(P<0.01), while the percentage of CD86 positive macrophages in each experimental group did not change obviously.Compared with the control group, the mRNA levels of FIZZ-1 and TGF-beta in GRP78 experimental groups were increased, and the levels of FIZZ-1 and TGF-beta in GRP78 40ng/ml group were increased significantly(P<0.01), while the mRNA levels of iNOS, IL-10 and ARG-1 in each group were not obviously changed.Conclusion: GRP78 may induce THP-1 into M2 macrophages.
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Key words:
- glucose-regulated protein 78 /
- THP-1 /
- macrophage /
- polarization
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