Abstract: Objective:To analyze the positive status of irregular antibody screening in hospitalized patients and the effect of blood transfusion in irregular antibodies positive patients.Method:The microcolumn gel technique was used to screen irregular antibody in the patients undergoing blood transfusion from January 2015 to December 2017,and the blood transfusion efficacy of irregular positive patients was analyzed.Result:14 706 cases were detected for irregular antibody screening,83 cases were positive for irregular antibodies,and the positive rate was 0.56%,including 9 male and 74 female.The positive rate in the patients with history of transfusion or pregnancy was 0.60%,which was significantly higher than that in the patients with no history of transfusion or pregnancy(χ²=4.26,P＜0.05).The number of irregular antibody screening of obstetrics and gynecology patients was the highest and the number of positive antibodies was also the highest.However,the positive rate was highest in blood rheumatology.Twelve patients with positive antibody screening were infused with red blood cells with ABO isotype and negative cross-matching test,11 cases were effective,the effective rate was 91.7%,and 2 cases had delayed hemolytic reaction.Conclusion:Irregular antibody screening for patients before transfusion clinical significance,especially for patients with a history of blood transfusion and pregnancy,may be a useful warning signal,as it can improve the efficacy of blood transfusion and ensure the safety of blood transfusion.