骨髓增生异常综合征患者骨髓单个核细胞免疫表型特点及临床意义

李萍; 欧阳建; 周荣富; 许景艳; 陈兵; 邵晓雁; 徐勇

doi:10.13201/j.issn.1004-2806.2012.04.009

骨髓增生异常综合征患者骨髓单个核细胞免疫表型特点及临床意义

- 南京大学医学院附属鼓楼医院血液科(南京, 210008)
基金项目:
江苏省卫生厅135开放课题(No:k0605)

南京市卫生局项目资助(No:ZKM06052,YKK08065)

详细信息

通讯作者: 欧阳建,E-mail:oy626@sina.com

中图分类号: R551.3

收稿日期: 2011-09-14

修回日期: 2011-11-14

Characteristics and clinical value of immunophenotyping of bone marrow cells for patients with myelodysplastic syndrome

- Department of Hematology, Affiliated DrumTower Hospital of Nanjing University Medical School, Nanjing, 210008, China

More Information

Corresponding author: OUYANG Jian, E-mail: oy626@sina.com

Received Date: 14 September 2011

Revised Date: 14 November 2011

摘要

摘要: 目的:探讨骨髓增生异常综合征(MDS)患者骨髓单个核细胞免疫表型特点及临床意义。方法:回顾性分析48 例MDS患者免疫表型,对比各亚型间免疫表型表达阳性率的高低,并评估其与IPSS积分的相关性。结果:48例MDS患者骨髓单个核细胞表达CD34、CD117、CD11b 、CD33、CD13为主,RAEB1及BAEB2患者CD34、CD117及早期髓系抗原CD33、CD13阳性表达率较RCMD患者增高(P＜0.05);骨髓原始细胞比例与CD34、CD117、CD13及CD33阳性表达率呈正相关;对这些患者进行IPSS积分系统评估,高危组CD34及CD117表达阳性率较中危1组升高(P＜0.05),CD34表达阳性率与IPSS积分呈正相关。结论:MDS患者进行骨髓单个核细胞免疫表型检测对病情评估及预后判断有重要价值。
- 骨髓增生异常综合征 /
- 免疫表型 /
- CD34
Abstract: Objective:To assess the clinical value of immunophenotyping for myelodysplastic syndrome (MDS) patients.Method:Analysis was performed on 48 bone marrow (BM) samples of MDS patients.Immunophenotyping was based on a primary gating of blast cells,monocytes,and granulocytes according to CD45 antigen expression and side scatter light diffraction.Immunophenotypic hierarchical clustering was performed to analyze the results.Result:The most discriminating markers were CD11b,CD13,CD33,CD34 and CD117.Positive expression of CD34,CD117,CD13 and CD33 was more frequent in RAEB1 and RAEB2 patients than in RCMD patients.The expression of CD34 was positively related to IPSS score.Conclusion: Immunophenotype of MDS patient is valuable for evaluating severity and predicting outcome.
- myelodysplastic syndrome /
- immunophenotyping /
- CD34

HTML全文

参考文献(8)

[1]	王建英,李晓明,李发菊,等.骨髓增生异常综合征患者骨髓细胞免疫表型研究及意义[J].中国实验血液学杂志,2002,10(1) :173-174.
[2]	[KG*1]JENSEN I M.Myelopoiesis in myelodysplasia evaluated by multiparameter flow cytometry[J].Leuk lymphoma,1995,20:17-25.
[3]	[KG*1]陈燕,隗佳.骨髓增生异常综合征(WHO分型)细胞的生物学特征及其意义[J].临床血液学杂志,2007,20(5):283-287.
[4]	[KG*1]ORFAO A,ORTUN F,DE SANTIAGO M.Immunophenotyping of acute leukemias and myelodysplastic syndromes[J].Cytometry(Part A 58A),2004:62-71.
[5]	[KG*1]MAYNADIE M,PICARD F,HUSSON B,et al.Immunophenotypic clustering of myelodysplastic syndromes[J].Blood,2002,100: 2349-2356.
[6]	[KG*1]吴雨洁,李建勇,仇海荣,等.四色流式细胞术分析骨髓增生异常综合征原始细胞免疫表型[J].中国实验血液学杂志,2006,14(1) :50-53.
[7]	[KG*1]MAY G,HEALY L,GALE K,et al.Functional analysis of the stem cell antigen CD34 in transgenic mice[J].Blood,1994,84:871-877.
[8]	[KG*1]FACKLER M,KRAUSE O,SMITH O M,et al.Full-length but not truncted CD34 inhibits hematopoietic cell differentiation of M1 cell[J].Blood,1995,85:3040-3047.