Differentiation and apoptosis of acute myelomonocytic leukemia cell line ACC247 induced by combination of ATRA and low dose Ara-C
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摘要: 目的:以急性粒-单核白血病细胞株ACC247为模型,采用全反式维甲酸(ATRA)、低剂量阿糖胞苷(Ara-C)单药及二者联合应用,观察其对ACC247细胞凋亡的影响。方法:用ATRA、低剂量Ara-C单药及二者联合处理ACC247细胞48 h后,分别用MTT法检测细胞增殖状况,Annexin V-FITC/PI双染检测细胞凋亡,倒置显微镜观察细胞形态判断分化。结果:单独ATRA作用于ACC247细胞,随着作用时间的增加可见细胞体积膨胀,细胞核固缩,抑制其增殖并促进其向成熟分化,进而凋亡,呈时间依赖性,剂量固定为1 μmol/L。单独低剂量Ara-C作用于ACC247白血病细胞,随着作用时间的增加可以使细胞数量减少,部分细胞核碎裂,细胞裂解,抑制其增殖并促进其凋亡,呈时间依赖性。ATRA和低剂量Ara-C二者联合应用,对ACC247细胞增殖抑制与诱导凋亡作用增强。结论:ATRA联合低剂量Ara-C可以抑制ACC247细胞增殖,促进其凋亡;联合用药的效果优于单用ATRA或Ara-C。Abstract: Objective:To explore the effects of all-trans retinoid acid (ATRA) and low dose cytosine arabinoside (Ara-C),either individually or in combination on the proliferation,apoptosis and differentiation of acute myelomonocytic leukemia cell line ACC247 cells.Methods:ACC247 cells were treated with ATRA and low dose Ara-C,either individually or in combination for different time.Cell proliferation was studied by MTT assay. Flow cytometry analysis of Annexin V-FITC/PI dual staining was used to examine apoptosis.Cell morphology was observed under the light microscope.Results:ATRA inhibited the proliferation and promoted differentiation of ACC247 cells in a time-dependent manner.Treatment of ACC247 cells by low dose Ara-C inhibited proliferation and induced apoptosis in a time-dependent manner.The combination of ATRA and Ara-C resulted in differentiation and apoptosis of ACC247 cells.Conclusion:Combinationof ATRA and low dose Ara-C can inhibit proliferation,induce differentiation and apoptosis of ACC247 cells.The data suggests that combination usage of ATRA and low dose Ara-C may be a useful treatment for acute myelomonocytic leukemia(M4).
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Key words:
- all-trans retinoid acid /
- cytosine arabinoside /
- ACC247cells /
- proliferation /
- apoptosis
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