1,25-（OH）<sub>2</sub>维生素D3对再生障碍性贫血患者T细胞亚群的影响

陆静涛; 杨建和; 章艳; 何金媛; 陈涛; 卢绪章

doi:10.13201/j.issn.1004-2806.2016.03.009

1,25-（OH）₂维生素D3对再生障碍性贫血患者T细胞亚群的影响

- 南京医科大学附属常州第二人民医院血液科(江苏常州, 213000)

详细信息

通讯作者: 卢绪章,E-mail:luxuzhang2008@163.com

中图分类号: R556.5

收稿日期: 2015-10-28

Effects of 1,25-(OH)₂ D3 on T cell subsets in patients with aplastic anemia

- Department of Hematology, Changzhou No.;

More Information

Corresponding author: LU Xuzhang, E-mail: luxuzhang2008@163.com

Received Date: 28 October 2015

摘要

摘要: 目的: 研究再生障碍性贫血(AA)患者外周血中的T细胞亚群变化与血清中1,25-(OH)₂维生素D3水平的关系。方法: 利用流式细胞仪检测15例AA患者和25例健康对照者外周血T细胞亚群的变化,并用ELISA法检测AA患者和健康对照者血清中1,25-(OH)₂维生素D3的水平。结果: AA患者血清中1,25-(OH)₂维生素D3水平明显低于健康对照者[(35.5±11.8) pmol/L:(51.8±12.6) pmol/L,P<0.05]。与健康对照者比较,AA患者外周血中CD4在淋巴细胞中比率无明显变化[(31.8±4.7)%:(33.6±4.2)%,P=0.217],但CD8在淋巴细胞中比率显著升高[(31.5±5.2)%:(24.8±3.8)%,P<0.01],故CD4/CD8比值降低(P<0.01);其中在CD4⁺细胞中,TH1/TH2比值较健康对照者明显升高[(4.09±1.05):(3.12±0.89),P=0.003]。结论: AA患者外周血清中1,25-(OH)₂维生素D3水平异常降低,可能影响AA患者CD4⁺T细胞的分化。
- 1,25-(OH)₂维生素D3 /
- 再生障碍性贫血 /
- T细胞亚群
Abstract: Objective: To study the effect of 1,25-(OH)₂ D3 on T cell subsets in patients with aplastic anemia (AA).Method: Fresh peripheral blood samples of 15 patients with AA and 25 normal controls were analyzed for the level of 1,25-(OH)₂ D3 with ELISA.The T cell subsets were detected by flow cytometry.Result: The median level of serum 1,25-(OH)₂ D3 of AA patients was lower than that of normal controls[(35.5±11.8) pmol/L vs.(51.8±12.6) pmol/L,P<0.05].There was no significant difference of the median number of CD4⁺ cells between AA patients and normal controls[(31.8±4.7)% vs.(33.6±4.2)%,P=0.217].The median number of CD8⁺ cells in AA patients was higher than that in normal controls[(31.5±5.2)% vs.(24.8±3.8)%,P<0.01].In CD4⁺ cells,the ratio of TH1/TH2 of AA patients was higher than that of normal controls[(4.09±1.05) vs.(3.12±0.89),P=0.003].Conclusion: The level of serum 1,25-(OH)₂ D3 is decreased in AA patients,which may affect the differentiation of CD4⁺ T cell in AA patients.
- 1,25-（OH）2 D3 /
- aplastic anemia /
- T cell subsets

HTML全文

参考文献(12)

[1]	Cutolo M,Pizzomi C,Sulli A.Vitamin D endocrine system involvement in autoimmune rheumatic diseases[J].Autoimmun Rev,2011,11:84-87.
[2]	Boonstra A,Barrat FJ,Crain C,et al.1alpha,25-Dihydroxyvitamin d3 has a direct effect on naive CD4(+) T cells to enhance the development of Th2 cells[J].J Immunol,2001,167:4974-4980.
[3]	Helming L,Bose J,Ehrchen J,et al.1alpha,25-Dihydroxyvitamin D3 is a potent suppressor of interferon gamma-mediated macrophage activation[J].Blood,2005,106:4351-4358.
[4]	Etten E,Stoffels K,Gysemans C,et al.Regulation of vitamin D homeostasis:implications for the immune system[J].Nutri Rev,2008,66:S125-S134.
[5]	Pelajo CF,Lopez-Benitez JM,Miller LC.Vitamin D and autoimmune rheumatologic disorders[J].Autoimmun Rev,2010,9:507-510.
[6]	Mahon BD,Wittke A,Wever V,et al.The targets of vitamin D depend on the differentiation and activation status of CD4 positive T cells[J].J Cell Biochem,2003,89:922-932.
[7]	Joseph RW,Bayraktar UD,Kim TK,et al.Vitamin D receptor upregulation in alloreactive human T cells[J].Hum Immunol,2012,73:693-698.
[8]	Wang Y,Zhu J,DeLuca HF.Where is the vitamin D receptor?[J].Arch Biochem Biophys,2012,523:123-133.
[9]	Hewison M.An update on vitamin D and human immumnity[J].Clin Endocrinol (Oxl),2012,76:315-325.
[10]	Hu X,Gu Y,Wang Y,et al.Increased CD4⁺ and CD8⁺ effector memory T cells in patients with aplastic anemia[J].Haematologica,2009,94:428-429.
[11]	Cantorna MT,Zhu Y,Fruicu M,et al.Vitamin D status,1,25-dihydroxyvitamin D3,and the immune system[J].Am J Clin Nutr,2004,80:1717-1720.
[12]	Cantorna MT,Mahon BD.Mounting evidence for vitamin D as an environmental factor affecting autoimmune disease prevalence[J].Exp Biol Med (Maywood),2004,229:1136-1142.