多虑平治疗慢性病贫血的机制研究

丁芳; 吴振添; 吴良华; 何云绮

doi:10.13201/j.issn.1004-2806.2016.03.012

多虑平治疗慢性病贫血的机制研究

- 1 福建医科大学附属闽东医院血液肿瘤科(福建福安, 355000);
- 2 遵义医学院第三附属医院血液科
基金项目:
宁德市科技计划项目(No:20150060)

福建省自然科学基金资助项目(No:闽科计[2015]15号)

详细信息

通讯作者: 何云绮,E-mail:943946824@qq.com

中图分类号: R556.9

收稿日期: 2015-12-25

An in vivo assay for doxepin in treating anemia of chronic disease

- 1 Department of Hematology and Oncology, Mindong Hospital, Fujian Medical University, Fu'an, 355000, China;
- 2 Department of Hematology, Zunyi Hospital of Zunyi Medical College

More Information

Corresponding author: HE Yunqi, E-mail: 943946824@qq.com

Received Date: 25 December 2015

摘要

摘要: 目的: 探讨多虑平治疗大鼠慢性病贫血(ACD)的机制。方法: 将24只大鼠按照随机对照原则共分为3个组:健康对照组、ACD模型组、多虑平治疗组,每组均为8只。药物治疗1周后采集新鲜血液,用自动化血液分析仪测定血常规,ELISA法检测血清中IL-6浓度;取肝组织,用RT-PCR法检测肝脏hepcidin mRNA表达量。结果: ACD模型组血清中IL-6水平、肝脏hepcidin mRNA表达量较健康对照组显著升高(P<0.01),且分别与血红蛋白呈线性负相关(r=-0.591、r=-0.759),肝脏hepcidin mRNA 表达量与血清IL-6水平呈线性正相关(r=0.619)。多虑平治疗组血清IL-6水平、肝脏hepcidin mRNA表达量较ACD模型组均显著下降(P<0.05),血红蛋白明显升高(P<0.01)。结论: 多虑平可改善大鼠ACD,作用机制可能与其通过抑制IL-6水平、降低hepcidin mRNA表达量有关。
- 慢性病贫血 /
- 多虑平 /
- IL-6 /
- hepcidin mRNA
Abstract: Objective: To elucidate the mechanism of doxepin in the treatment of mouse anemia of chronic disease (ACD).Method: A total of 24 male SD rats were randomly divided into 3 groups:healthy control group,ACD model group and doxepin treatment group,each group had 8 rats.After 7 days of treatment,peripheral blood was collected for counting hemoglobin (Hb) by automated blood analyzers and the level of serum IL-6 was detected by ELISA,liver tissues were also collected for assessment of hepcidin mRNA by RT-PCR.Result: Compared to healthy control group,serum IL-6,hepatic hepcidin mRNA expression in ACD model group were significantly increased (P<0.01),and both were negatively correlated with Hb (r=-0.591,r=-0.759).There was a positive correlation betweenhepatic hepcidin mRNA expression and IL-6 level (r=0.619).Compared to ACD model group,serum IL-6 levels and hepatic hepcidin mRNA expression in the doxepin treatment group were significantly decreased (P<0.05),and Hb was increased (P<0.01).Conclusion: Doxepin should alleviate ACD in rats.It might be interpreted by decreasing hepcidin mRNA expression and inhibiting the secretion of IL-6.
- anemia of chronic disease /
- doxepin /
- interleukin-6 /
- hepcidin mRNA

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