Th22细胞在慢性粒细胞白血病患者中的临床研究

陈萍; 胥莉; 王敏; 李大启; 纪春岩; 马道新

doi:10.13201/j.issn.1004-2806.2016.03.016

Th22细胞在慢性粒细胞白血病患者中的临床研究

- 1 山东大学附属济南市中心医院血液内科(济南, 250013);
- 2 山东大学齐鲁医院血液内科

详细信息

通讯作者: 马道新,E-mail:daoxinma@sdu.edu.cn

中图分类号: R733.72

收稿日期: 2015-05-07

Clinical study of Th22 cells in patients with chronic myeloid leukemia

- 1 Department of Hematology, Jinan Central Hospital, Affiliated to Shangdong University, Jinan, 250013, China;
- 2 Department of Hematology, Qilu Hospital of Shangdong University

More Information

Corresponding author: MA Daoxin, E-mail: daoxinma@sdu.edu.cn

Received Date: 07 May 2015

摘要

摘要: 目的: 研究慢性粒细胞白血病(CML)患者外周血中Th22细胞的比例、相关转录因子芳香烃受体(AHR)的表达水平及细胞因子IL-22的浓度,探讨Th22细胞在CML免疫机制中的作用。方法: 应用流式细胞术检测Th22细胞在33例CML患者(初诊15例,伊马替尼治疗后的CML-CP 18例)外周血中的比例,实时荧光定量PCR技术检测CML患者Th22相关转录因子AHR mRNA的表达水平,ELISA法检测细胞因子IL-22的浓度。同时以15例正常者作为对照。结果: CML初诊患者外周血的Th22细胞比例(0.43%±0.23%)明显低于伊马替尼治疗后的CML-CP患者(3.19%±1.76%,P<0.05)和对照者(2.58%±0.98%,P<0.05)。CML初诊患者外周血单个核细胞的AHR基因转录水平(0.2129±0.1746)与伊马替尼治疗后的CML-CP患者(0.8171±0.3887,P<0.05)和对照者(0.4681±0.1804,P<0.05)比较均明显降低。伊马替尼治疗后的CML-CP患者外周血AHR mRNA表达水平较对照者显著升高(P<0.05)。CML-CP患者的外周血IL-22浓度较对照者显著升高[(100.94±18.48) pg/ml:(85.14±13.46) pg/ml,P<0.05]。Th22细胞的比例与CML患者外周血白细胞计数及BCR-ABL%均呈负相关。结论: Th22细胞下调,Th22免疫功能受损可能有助于CML的发生和发展。
- 白血病,粒细胞,慢性 /
- Th22细胞 /
- IL-22
Abstract: Objective: To explore the ratio of Th22 cells,the expression of transcription factors AHR and their related cytokines IL-22 in peripheral blood of chronic myeloid leukemia (CML) patients,and to further investigate the role of Th22 cells in hematological tumor immune mechanisms.Method: The proportions of Th22 cells in peripheral blood of 33 patients with CML were evaluated by flow cytometry analysis.AHR mRNA expressions were examined by RT-PCR.The levels of cytokines IL-22 were measured by ELISA.Fifteen normal subjects were used as controls.Result: The ratio of Th22 cells was significantly decreased in newly-diagnosed CML (CML-NP) patients (0.43%±0.23%) than those in chronic phase CML (CML-CP) patients (3.19%±1.76%,P<0.05) and controls (2.58%±0.98%,P<0.05).The expression of AHR was significantly decreased in CML-ND patients (0.212 9±0.174 6) compared with CML-CP patients (0.817 1±0.388 7,P<0.05) and controls (0.468 1±0.180 4,P<0.05).The mRNA level of AHR was increased in CML-CP patients than that in controls (P<0.05).The level of IL-22 in CML-CP patients was slightly higher than controls[(100.94±18.48) pg/ml vs.(85.14±13.46) pg/ml,P<0.05].There were significantly negative correlations between Th22 cells and peripheral white blood cell counts and BCR-ABL%.Conclusion: Down-regulation of Th22 cellular immunity and impaired Th22 immune function may contribute to the occurrence and development of CML.
- chronic myeloid leukemia /
- Th22cells /
- IL-22

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