蛇床子素通过抑制PI3K/AKT信号通路诱导K562细胞凋亡和增殖抑制

潘莉萍; 郭静明; 袁伟

doi:10.13201/j.issn.1004-2806.2016.03.017

蛇床子素通过抑制PI3K/AKT信号通路诱导K562细胞凋亡和增殖抑制

- 三峡大学第一临床医学院宜昌市中心人民医院血液科(湖北宜昌, 443000)
基金项目:
2010年宜昌市科研项目(No:A11301-38)

详细信息

通讯作者: 袁伟,E-mail:1042157787@qq.com

中图分类号: R331.1

收稿日期: 2015-03-29

Osthole induced proliferation inhibition and apoptosis of K562 cells by suppressing PI3K/AKT signal pathway

- Department of Hematology, Yichang Central Hospital, Yichang, 443000, China

More Information

Corresponding author: YUAN Wei, E-mail: 1042157787@qq.com

Received Date: 29 March 2015

摘要

摘要: 目的: 研究蛇床子素对人慢性髓系白血病细胞K562增殖和凋亡的影响,并探讨其相关作用机制。方法: 培养髓系白血病细胞K562,分别用不同浓度的蛇床子素处理(0、5、10、20 μg/ml)。细胞培养48 h后,利用MTT法检测K562细胞增殖;流式检测K562细胞凋亡;Western blot法检测PI3K、p-AKT、AKT、Bax、Bcl-2和Cleavage-Caspase3表达。结果: 蛇床子素可以呈浓度依赖性的诱导K562细胞增殖抑制和凋亡,上调Bax和Cleavage-Caspase3表达,下调PI3K、p-AKT和Bcl-2,对AKT表达无明显影响。结论: 蛇床子素可通过抑制PI3K/AKT信号通路而诱导K562细胞增殖抑制和凋亡。
- 蛇床子素 /
- K562细胞 /
- 增殖 /
- 凋亡 /
- PI3K/AKT
Abstract: Objective: To study the influence and potential mechanism of osthole in the proliferartion and apoptosis of human leukemia K562 cells.Method: The K562 cell was cultured in the DMEM.MTT method was used to evaluate the proliferation effect of K562 and the apoptosis of K562 cells were evaluated by flow cytometric analysis.The expression of PI3K,p-AKT,AKT,Bax,Bcl-2 and Cleavage-Caspase3 were detected by Western blot.Result: The osthole treatment can significantly attenuate the proliferartion rate of K562 cell,the expression of PI3K,p-AKT and Bcl-2 and increase the apoptosis rate of K562 cell,the expression of Bax and Cleavage-Caspase3.Wherefore,it had no influence on the expression of AKT.Conclusion: Osthole can induce the apoptosis and inhibit the proliferation of human leukemia K562 cells through suppressing PI3K/AKT signaling pathway.
- osthole /
- K562cell /
- proliferation /
- apoptosis /
- PI3K/AKT

HTML全文

参考文献(3)

[1]

Zuckerman T,Rowe JM.Pathogenesis and prognostication in acute lymphoblastic leukemia[J].F1000Prime Rep,2014,6:59.

[2]

Xu XM,Zhang Y,Qu D,et al.Osthole suppresses migration and invasion of A549 human lung cancer cells through inhibition of matrix metalloproteinase-2 and matrix metallopeptidase-9 in vitro[J].Mol Med Rep,2012,6:1018-1022.

[3]

Kang Q,Yan S.Piperlongumine reverses doxorubicin resistance through the PI3K/Akt signaling pathway in K562/A02 human leukemia cells[J].Exp Ther Med,2015,9:1345-1350.

施引文献

资源附件(0)

访问统计