地中海贫血患者氧化应激与铁代谢的关系

刘姣娣; 雷宇; 黄语妹; 赖永榕

doi:10.13201/j.issn.1004-2806.2017.01.006

地中海贫血患者氧化应激与铁代谢的关系

- 广西医科大学第一附属医院血液内科(南宁, 530000)
基金项目:
国家自然科学基金（No：81360085）；2012年度高等学校博士学科点专项科研基金资助课题（No：20124503110009）

详细信息

通讯作者: 赖永榕,E-mail:laiyongrong@hotmail.com

中图分类号: R556

收稿日期: 2016-12-02

The relationship between oxidative stress and iron metabolism in patients with thalassemia

- Department of Hematology, the First Affiliated Hospital of Guangxi Medical University, Nanning, 530000, China

More Information

Corresponding author: LAI Yongrong, E-mail: laiyongrong@hotmail.com

Received Date: 02 December 2016

摘要

摘要: 目的：了解地中海贫血患者体内的氧化应激及铁代谢水平，并探讨两者之间的相关性。方法：选取30例重型地中海贫血患者（A组）及60例血红蛋白H病患者（B组）作为观察组，并选取同期到我院体检的30例健康人群（C组）作为对照组。采用丙二醛（MDA）、超氧化物歧化酶（SOD）、维生素C （VC）及总抗氧化能力（TAC）评价氧化应激水平；采用血清铁蛋白（SF）和肝脏铁浓度（LIC）评估体内铁状态；采用促红细胞生成素（EPO）和转化生长因子-15（GDF15）评估铁代谢水平。测定所有研究对象血清的MDA、SOD、VC、TAC、SF、EPO、GDF15含量，并对其中5例A组患者及24例B组患者采用磁共振测定LIC，且探讨氧化应激与铁代谢的相关性。结果：与C组相比，A组血清MDA、SOD升高，VC下降，差异均有统计学意义（P<0.01）；与C组相比，B组血清MDA升高，VC下降，差异均有统计学意义（P<0.01）；A组及B组血清TAC均降低，但与C组相比，差异均无统计学意义。观察组血清MDA、SOD与SF、LIC呈正相关（P<0.05），血清VC、TAC与SF、LIC呈负相关（P<0.05）；观察组血清MDA与SOD、EPO、GDF15呈正相关（P<0.05），与VC呈负相关（P<0.05）。结论：地中海贫血患者存在铁过载和氧化应激，而且氧化应激与铁代谢有关。
- 贫血,地中海 /
- 铁代谢 /
- 氧化应激
Abstract: Objective: To investigate the level of iron metabolism,oxidative stress in thalassemia patients,and to explore the correlation between oxidative stress and iron metabolism.Method: We chose 30 outpatients with thalassemia major (group A) and 60 outpatients with hemoglobin H disease (group B) as observed subjects,and 30 healthy people (group C) as control group.We evaluated oxidative stress by using the serum malondialdehyde (MDA),superoxide dismutase (SOD),vitamin C (VC) and total antioxidant capacity (TAC).And we determined serum ferritin and liver iron concentration (LIC) to evaluate the iron condition.We chose erythropoietin (EPO) and grow differentiation factor-15 (GDF15) as marks to evaluate iron metabolism.We detected the serum MDA,SOD,VC,TAC,hepcidin,EPO and GDF15 in all of controls and observations.We also detected the LIC by MRI in 5 patiens with thalassemia major and 24 patients with Hb H disease.And we explored the relationship between iron metabolism and oxidative in thalassemia patients.Result: There were significantly increased levels of MDA,SOD and decreased level of VC in serum in group A,when compared with those in group C (P<0.01).There were also significantly increased levels of MDA and decreased level of VC in serum in group B,when compared with those in group C (P<0.01).The levels of serum TAC were decreased in the group A and group B,but there were no significant differences when compared with group C.Meanwhile,the levels of serum MDA,SOD were positively correlated with SF,LIC (P<0.05),the levels of serum VC,TAC were negatively correlated with SF,LIC (P<0.05) in the observed subjects.The level of serum MDA was positively correlated with SOD,EPO and GDF15 (P<0.05),and negatively correlated with VC in the observed subjects (P<0.05).Conclusion: It demonstrates the presence of oxidative stress and iron overload in thalassemia patients.Moreover,the related metabolic pathway of oxidative stress is active with iron metabolism.
- thalassemia /
- iron metabolism /
- oxidative stress

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