环孢素A对脐带血干细胞和T淋巴细胞的影响

燕法红; 居瑞雪; 赵云霞; 毛晓臻; 林彩莲

doi:10.13201/j.issn.1004-2806.2021.08.008

环孢素A对脐带血干细胞和T淋巴细胞的影响

- 1. 潍坊医学院第一附属医院潍坊市人民医院血液内科(山东潍坊, 261041);
- 2. 潍坊医学院第一附属医院潍坊市人民医院检验科;
- 3. 潍坊医学院第一附属医院潍坊市人民医院产科
基金项目:
山东省医药卫生科技发展计划项目(No:2016WS0644)

详细信息

通讯作者: 林彩莲,E-mail:1135081139@qq.com

中图分类号: R329.2,R557

收稿日期: 2020-12-09

Impact of cyclosporin A on umbilical cord blood stem cell and T lymphocyte

- 1. Department of Hematology, First Affiliated Hospital of Weifang Medical College, Weifang People's Hospital, Weifang, 261041, China;
- 2. Department of Laboratory Medicine, First Affiliated Hospital of Weifang Medical College, Weifang People's Hospital;
- 3. Department of Obstetrics, First Affiliated Hospital of Weifang Medical College, Weifang People's Hospital

More Information

Corresponding author: LIN Cailian, E-mail: 1135081139@qq.com

Received Date: 09 December 2020

摘要

摘要: 目的:探讨环孢素A(CsA)对脐带血干细胞及T淋巴细胞的影响。方法:采集10例产妇的脐带血,每例脐带血分为5份,每份2 mL,设对照组(无CsA)、CsA 5μmol/L组、CsA 10μmol/L组、CsA 20μmol/L组、CsA 40μmol/L组,用血细胞分析仪检测各组的白细胞数,应用流式细胞术检测各组的干细胞(CD34⁺细胞)、T淋巴细胞(CD3⁺细胞)占CD45⁺细胞的比例,并分别计算出干细胞、T淋巴细胞的数值。采用配对样本t检验,分别比较不同浓度的CsA组与对照组在白细胞数、干细胞数、T淋巴细胞数方面的差别。以线性相关分析各组白细胞数与T淋巴细胞数之间的相关性。结果:(1)白细胞数:CsA 5μmol/L组、CsA 10μmol/L组、CsA 20μmol/L组、CsA 40μmol/L组均低于对照组(P<0.05)。(2)干细胞数:CsA 5μmol/L组、CsA 10μmol/L组、CsA 20μmol/L组、CsA 40μmol/L组与对照组比较差异无统计学意义(P>0.05)。(3)T淋巴细胞数:高浓度CsA组(10~40μmol/L)与对照组比较,T淋巴细胞数均降低(P<0.01);低浓度CsA组T淋巴细胞数与对照组差异无统计学意义(P=0.133)。(4)各组内白细胞数与T淋巴细胞数之间无相关性(P>0.05)。结论:CsA无法提高脐带血中的干细胞数,可降低脐带血中的白细胞数和T淋巴细胞数。
- 环孢素A /
- 脐带血 /
- 干细胞 /
- T淋巴细胞 /
- 白细胞
Abstract: Objective: To explore the impact of cyclosporin A on umbilical cord blood stem cell and T lymphocyte.Methods: Ten umbilical cord blood units were collected and each was divided into five groups including control group(without CsA), CsA 5 μmol/L group, CsA 10 μmol/L group, CsA 20 μmol/L group and CsA 40 μmol/L group with two milliliters for each group. White blood cell(WBC) was measured using automated hematology analyzer. CD34⁺and CD3⁺cell ratios were analyzed using flow cytometry. CD34⁺and CD3⁺cell counts were further calculated. Paired sample t test was used to compare difference of WBC, stem cell, T lymphocyte between each CsA group and control group respectively. Relationships between WBC and T lymphocyte in each group were analyzed using linear correlation.Results:①WBC count was lower in each CsA group compared to control group(P<0.05).②There was no difference of stem cell count between each CsA group and control group(P>0.05).③T lymphocyte count of CsA 10-40 μmol/L group was all lower than that of control group(P<0.01). There was no difference of T lymphocyte count between CsA 5 μmol/L group and control group(P=0.133).(4)There was no relationship between WBC and T lymphocyte in each group(P>0.05).Conclusion: CsA can not improve stem cell of umbilical cord blood and can reduce WBC and T lymphocyte.
- cyclosporin A /
- umbilical cord blood /
- stem cell /
- T lymphocyte /
- white blood cell

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参考文献(21)

[1]	Huang X,Trinh T,Aljoufi A,et al.Hypoxia Signaling Pathway in Stem Cell Regulation:Good and Evil[J].Curr Stem Cell Rep,2018,4(2):149-157.
[2]	Mohammadali F,Abroun S,Atashi A.Mild hypoxia and human bone marrow mesenchymal stem cells synergistically enhance expansion and homing capacity of human cord blood CD34+ stem cells[J].Iran J Basic Med Sci,2018,21(7):709-716.
[3]	Shin DY,Huang X,Gil CH,et al.Physioxia enhances T-cell development ex vivo from human hematopoietic stem and progenitor cells[J].Stem Cells,2020.38(11):1454-1466.
[4]	Chabi S,Uzan B,Naguibneva I,et al.Hypoxia Regulates Lymphoid Development of Human Hematopoietic Progenitors[J].Cell Rep,2019,29(8):2307-2320.
[5]	Mantel CR,O'Leary HA,Chitteti BR,et al.Enhancing Hematopoietic Stem Cell Transplantation Efficacy by Mitigating Oxygen Shock[J].Cell,2015,161(7):1553-1565.
[6]	Zhang CX,Cheng Y,Liu DZ,et al.Mitochondria-targeted cyclosporin A delivery system to treat myocardial ischemia reperfusion injury of rats[J].J Nanobiotechnology,2019,17(1):18.
[7]	Alexopoulos P,Panoutsopoulou K,Vogiatzis G,et al.Combined Treatment With Exenatide and Cyclosporine A or Parstatin 1-26 Results in Enhanced Reduction of Infarct Size in a Rabbit Model[J].J Cardiovasc Pharmacol,2017,70(1):34-41.
[8]	Ikeda G,Matoba T,Nakano Y,et al.Nanoparticle-Mediated Targeting of Cyclosporine A Enhances Cardioprotection Against Ischemia-Reperfusion Injury Through Inhibition of Mitochondrial Permeability Transition Pore Opening[J].Sci Rep,2016,6:20467.
[9]	黄光银,文星,付世全,等.环孢素A联合缺血适应对于急性ST段抬高型心肌梗死患者再灌注损伤的影响[J].现代中西医结合杂志,2019,28(23):2528-2532,2536.
[10]	Okahara A,Koga JI,Matoba T,et al.Simultaneous targeting of mitochondria and monocytes enhances neuroprotection against ischemia-reperfusion injury[J].Sci Rep,2020,10(1):14435.
[11]	Forsse A,Nielsen TH,Nygaard KH,et al.Cyclosporin A ameliorates cerebral oxidative metabolism and infarct size in the endothelin-1 rat model of transient cerebral ischaemia[J].Sci Rep,2019,9(1):3702.
[12]	Fakharnia F,Khodagholi F,Dargahi L,et al.Prevention of Cyclophilin D-Mediated mPTP Opening Using Cyclosporine-A Alleviates the Elevation of Necroptosis,Autophagy and Apoptosis-Related Markers Following Global Cerebral Ischemia-Reperfusion[J].J Mol Neurosci,2017,61(1):52-60.
[13]	Serkova N,Donohoe P,Gottschalk S,et al.Comparison of the effects of cyclosporin a on the metabolism of perfused rat brain slices during normoxia and hypoxia[J].J Cereb Blood Flow Metab,2002,22(3):342-352.
[14]	Lemos SV Neto,Vianna IG,Castiglia YM,et al.Cyclosporine A attenuates apoptosis and necrosis after ischemia-reperfusion-induced renal injury in transiently hyperglycemic rats[J].Acta Cir Bras,2017,32(3):203-210.
[15]	Yang H,Li R,Zhang L,et al.p53-cyclophilin D mediates renal tubular cell apoptosis in ischemia-reperfusion-induced acute kidney injury[J].Am J Physiol Renal Physiol,2019,317(5):F1311-F1317.
[16]	Lemoine S,Pillot B,Augeul L,et al.Dose and timing of injections for effective cyclosporine A pretreatment before renal ischemia reperfusion in mice[J].PLoS One,2017,12(8):e0182358.
[17]	程小欢,袁佳仪,荣伽玲,等.老年心肺慢性疾病中氧化应激指标的变化差异分析[J].临床血液学杂志,2020,33(6):402-406.
[18]	Oliveira ACC,Módolo NSP,Domingues MAC,et al.Effects of cyclosporine on ischemia-reperfusion injuries in rat kidneys.An experimental model[J].Acta Cir Bras,2019,34(8):e201900806.
[19]	Upadhaya S,Madala S,Baniya R,et al.Impact of cyclosporine A use in the prevention of reperfusion injury in acute myocardial infarction:A meta-analysis[J].Cardiol J,2017,24(1):43-50.
[20]	Ottani F,Latini R,Staszewsky L,et al.Cyclosporine A in Reperfused Myocardial Infarction:The Multicenter,Controlled,Open-Label CYCLE Trial[J].J Am Coll Cardiol,2016,67(4):365-374.
[21]	Ederoth P,Dardashti A,Grins E,et al.Cyclosporine before Coronary Artery Bypass Grafting Does Not Prevent Postoperative Decreases in Renal Function:A Randomized Clinical Trial[J].Anesthesiology,2018,128(4):710-717.