2种方法检测与痰涂片阴性肺结核患者诊断效能的关系研究

黄琦; 吴婷婷; 孔晓明; 周翔

doi:10.13201/j.issn.1004-2806.2021.08.010

2种方法检测与痰涂片阴性肺结核患者诊断效能的关系研究

- 溧阳市人民医院检验科(江苏溧阳, 213300)
基金项目:
常州市科研计划项目(No:WZ201730)

详细信息

通讯作者: 周翔,E-mail:312799781@qq.com

中图分类号: R521

收稿日期: 2021-02-01

Study on the relationship between two methods and the diagnostic efficiency of sputum smear negative pulmonary tuberculosis patients

- Department of Laboratory Medicine, Liyang People's Hospital, Liyang, 213300, China

More Information

Corresponding author: ZHOU Xiang, E-mail: 312799781@qq.com

Received Date: 01 February 2021

摘要

摘要: 目的:研究利福平耐药实时荧光定量核酸扩增技术(Xpert MTB/RIF)结合结核感染T细胞试验(T-SPOT)2种方法检测与痰涂片阴性肺结核患者诊断效能的关系。方法:收集2020年1月—2020年10月就诊的呼吸系统疾病患者212例,分为对照组(102例)和结核组(110例)。对所有患者进行Xpert MTB/RIF以及T-SPOT检查,分析2种检测方法对结核病以及涂片阴性肺结核的诊断效能之间的差异。结果:采用Xpert MTB/RIF、T-SPOT、涂片及培养4种方法同时对结核组和对照组进行检测,不同方法结果比较差异有统计学意义(P<0.001);Xpert MTB/RIF、T-SPOT、联合检测的灵敏度分别为59.09%、64.49%及83.46%。联合检测诊断对肺结核患者的诊断灵敏度显著高于单独检测(P<0.05);通过联合诊断效能分析,联合诊断对涂阴肺结核患者的诊断灵敏度显著高于单独检测。结论:Xpert MTB/RIF结合T-SPOT检测对于痰涂片阴性肺结核患者的诊断灵敏度高,建议临床应用推广。
- 荧光定量核酸扩增技术 /
- 结核感染T细胞试验 /
- 肺结核 /
- 联合诊断
Abstract: Objective: To study the relationship between xpert MTB/RIF combined with T-SPOT detection and diagnostic efficiency of smear negative pulmonary tuberculosis patients.Methods: A total of 212 patients with respiratory diseases hospitalized in the respiratory department of our hospital from January 2020 to October 2020 were collected and divided into control group(n=102) and tuberculosis group(n=110). All patients were examined by xpert MTB/RIF and T-SPOT respectively to analyze the difference between the two diagnostic methods in the diagnosis of tuberculosis and smear negative tuberculosis.Results: The sensitivity of xpert MTB/RIF, T-SPOT, smear and culture were 59.09%, 64.49% and 83.46%, respectively. The diagnostic sensitivity of combined detection in patients with pulmonary tuberculosis was significantly higher than that of single detection(P<0.05).The diagnostic sensitivity of combined diagnosis in patients with smear negative pulmonary tuberculosis was significantly higher than that of single detection.Conclusion: The sensitivity of xpert MTB/RIF combined with T-SPOT detection in the diagnosis of smear negative pulmonary tuberculosis patients is significantly increased, and it is recommended to be popularized in clinical practice.
- Xpert MTB/RIF /
- T-SPOT /
- tuberculosis /
- joint diagnosis

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