Thomsen-Friedenreich activation of erythrocytes in infants leads to cross-matching incompatibility
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摘要: 检测和分析交叉配型中继发性侧向错配患儿的血清学样本。采用ABO、RhD血型鉴定、Coombs试验、交叉配型，新生儿血浆和多发性凝集红细胞特异性试验。结果发现儿童红细胞与新生儿血浆不凝集，但AB型血浆和成人花生凝集素发生凝集。提示儿童红细胞T抗原活化导致交叉配型亚侧错配。Abstract: To detect and analyze the serological samples of a child with secondary lateral mismatch in cross matching. ABO and RhD blood group identification, Coombs test, cross matching test were used. The specificity tests of neonatal plasma and multiple agglutinated erythrocytes were performed. Erythrocytes of children and plasma of neonates did not agglutinate, but plasma of AB type and peanut agglutinin of adults. T-antigen activation of erythrocytes in children leads to sublateral mismatch of cross matching.
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Key words:
- Thomsen-Friedenreich activated /
- neuraminidase /
- sialic acid /
- severe sepsis
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表 1 患儿及供血者血型鉴定结果
样本 单克隆抗血清 Ctl 试剂红细胞 抗-A 抗-B 抗-D Ac Bc Oc 患者 0 0 4+ 0 4+ 4+ 0 供血者 0 0 4+ 0 4+ 4+ 0 
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表 2 患儿与5份不同供血者交叉配血结果
血样 主侧 次侧 盐水介质 抗人球蛋白介质 聚凝胺介质 盐水介质 抗人球介质 聚凝胺介质 供血者1 0 0 0 2+ 2+s 2+ 供血者2 0 0 0 2+ 2+ 2+ 供血者3 0 0 0 2+ 2+ 2+ 供血者4 0 0 0 1+s 2+ 2+ 供血者5 0 0 0 2+ 2+ 2+ 自身反应 0 0 0 / / /
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表 4 多重凝集红细胞确认
红细胞悬液(5%) 新生儿血浆(AB型3人份) AB型血浆(AB型5人份) 花生水 患儿血浆 患儿样本 - 2+ 4+ - 阳性对照 - 2+ 4+ - 阴性对照 - - - - 阳性对照：神经氨酸酶处理的O型红细胞(5%); 阴性对照：正常O型献血者红细胞(5%)。
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