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摘要: 目的：观察细胞表面分化抗原CD117和CD34在急性白血病中的表达及意义。方法：采用三色流式细胞术，CD45/SSC双参数散点图设门方法，对34例急性白血病患者[急性淋巴细胞白血病（ALL）9例和急性髓细胞白血病（AML）25例]骨髓白血病细胞的免疫分型及CD117和CD34的表达进行检测。结果：CD117在ALL和AML中表达率分别为0和76.0%（19/25），差异有统计学意义（P=0.000）。CD34在ALL和AML中表达率分别为77.8%（7/9）和60.0%（15/25），差异无统计学意义（P=0.815）。在AML-M3亚型中，CD34表达率为16.7%（1/6），显著低于非M3亚型73.7%（14/19）（P=0.007）。结论：CD117检测有助于ALL与AML的鉴别诊断，CD34检测有助于区分AML-M3亚型和非M3亚型。Abstract: Objective: To explore the significance and expression of CD34 and CD117 on different types of acute leukemia cells.Method: The expression of CD34 and CD117 in acute leukemia cells from 34 patients (including 9 ALL and 25 AML) were detected by flow cytometry.Result: The positive rate of CD117 was significantly lower in ALL patients than that in AML patients (0 vs 76.0%,P=0.000).There was no significant difference in the positive rate of CD34 between ALL and AML patients (77.8% vs 60.0%,P=0.815).The positive rate of CD34 in non-M3 subtype was significantly higher than that in AML-M3 subtype (73.7% vs 16.7%,P=0.007).Conclusion: CD117 may be used as an immunologic marker for AML.CD34 may be used to distinguish the diagnosis between AML-M3 subtype and non-M3 subtype.
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Key words:
- acute leukemia /
- flow cytometry /
- CD117 /
- CD34
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