Abstract: Objective: To retrospectively study the data of the donors whose ABO blood typing discrepancy and estimate the discarded blood caused by ABO blood typing discrepancy in Hebei Blood Center.Method: A total 241 blood samples of donors were collected in Hebei Blood Center.ABO blood typing and irregular antibody screening test were performed to find the cause of the discrepancy.Result: In 241 cases of ABO blood typing discrepancy,ABO subtype were 69 cases,accounting for 28.63% of total samples;false aggregation or condensation 27 cases,accounting for 11.20%;antibody screening positive 48(19.92%);antibodies to abate or missing 78(32.37%) and normal ABO blood typing 19 cases(7.88%).Conclusion: ABO blood typing discrepancy was mainly caused by ABO subtype,false aggregation or condensation,antibody screening positive and antibodies to abate or missing.For these blood with ABO blood typing discrepancy how to deal with in our country had no uniform standard,generally to discard.Maybe some of them could be used for clinical purposes,which would be discussed together.