Abstract: Objective:To evaluate the accuracy and reliability of ORTHO AutoVue Innova fully automated blood grouping analyzer in blood grouping.Method:572 patients’ ABO and RhD blood groups were examined by conventional tube test and the fully automated blood grouping analyzer from July to August 2010,and the two methods’ results were compared to calculate their concordance rate. 10314 pations’ ABO and RhD blood groups were tested by the fully automated blood grouping analyzer from September 2010 to April 2011,and the analyzer’s errors were recorded and analyzed.Result:In the control trial,there were 10 samples’ cards could not be interpreted by the analyzer. The analyzer’s results were the same as the tube method in the other 562 samples. The analyzer’s accuracy rate was 98.8%,and its failure rate was 1.2%,while its error rate was 0 in ABO blood grouping. Its accuracy rate was 100% in RhD blood grouping.Conclusion:The analyzer was reliable in blood grouping, because its standard and automated operation process might eliminate artificial errors. Its results can be stored permanently, so it may be easy to make inquiry and medical evidence.