Abstract: Objective:To investigate the antimicrobial resistance of gram negative bacilli (g-b) causing lower respiratory tract infection in stroke patients and provide the reference for clinical anti-infection therapy.Method:Referring to National Guide to Clinical Laboratory Procedures, 212 g-b were identified by the routine methods. The susceptibility tests were performed by K-B methods. And the testing results were assessed according to CLSI 2009-2011 breakpoints. Result:The main pathogenic bacteria causing lower respiratory tract infection in stroke patients were Pseudomonas aeruginosa (26.4%), K.Pneunmoniae (21.2%), Escherichia coli (16.0%), A.baumannii (14.2%) and Stenotrophomonos maltophilia (9.9%). The severe antimicrobial resistance occurred in the above strains. The antibiotics with the highest resistance rates was SMZ-TMP (62.5% -70.0%). The resistance rates of imipenem and meropenem against Pseudomonas aeruginosa and Acinetobacter baumannii varied between 17.9% and 26.7%.Conclusion:The antimicrobial resistance of g-b causing lower respiratory tract infection in stroke patients was severe. Targeted interventions should be taken to restrain the adverse trends of the rapid growth of bacterial resistance.