Abstract: Objective:To investigate bacterial species and antibiotical resistance of pathogenic bacteria causing pulmonary infection in patients with chronic obstructive pulmonary diseases (COPD) and provide the referrence for clinically reasonable use of antibiotics. Method:Referring to National guide to clinical laboratory procedures, pathogenic bacteria were cultured and identified by the routine methods. The antibiotical susceptibility testings were performed by K-B method. The susceptibility testings results were assessed according to the latest CLSI breakpoints and analyzed by WHONET 5.5 software. Result:Pathogens were mainly Gram-negative bacilli (71.0%). All pathogens had a more serious resistance to commonly used antimicrobial agents. Detection rate of methicillin-resistant staphylococcus aureus (MRSA) was 41.9%. That of ESBL-producing bacteria was 44.4%. Carbapenem-resistant Pseudomonas aeruginosa and Acinetobacter baumannii arrived at 13.3% and 20.0%, respectively. Conclusion:The antibiotical resistance of pathogenic bacteria causing pulmonary infection in patients with COPD was increasing. We should strengthen monitoring and controlling of it.