Effect of antithymocyte globulin and umbilical cord-derived mesenchymal stem cells on patients with severe aplastic anemia
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摘要: 目的：探索脐带间充质干细胞（UC-MSCs）与抗胸腺细胞球蛋白（ATG）联合治疗重型再生障碍性贫血患者的可行性、安全性和作用机制。方法：采用UC-MSCs联合ATG治疗1例重型再生障碍性贫血Ⅰ型，观察血常规、网织红细胞、骨髓细胞学变化，采用流式细胞仪检测干扰素（IFN）-γ、调节T细胞（Treg）变化。结果：UC-MSCs输注后2周，患者血细胞计数、网织红细胞计数开始上升，骨髓增生活跃，粒系和红系增生，输血间隔时间延长至每11~12 d 1次，IFN-γ水平下降，Treg细胞水平上升；无呼吸道感染及肝肾功能异常。结论：UC-MSCs与ATG联合治疗有利于促进再生障碍性贫血患者骨髓造血能力的恢复，无不良反应发生。Abstract: Objective: To study the effect of umbilical cord-derived mesenchymal stem cells (UC-MSCs) combined with antithymocyte globulin (ATG) on patients with severe aplastic anemia(SAA).Method: A patient of SAA-Ⅰ was treated with ATG and UC-MSCs.Blood cell count,reticulocyte and marrow hyperplasia were performed.Flow cytometry was used to determine IFN-γ and regulatory T cells (Treg).Result: The blood cell count,reticulocyte and Treg of the patient elevated,bone marrow hyperplasia became more active than before,the interval time of blood transfusion extended and the level of IFN-γ descended.There was no respiratory infection,abnormality of the hepatic and renal function.Conclusion: ATG and UC-MSCs can improve the hematogenesis of bone marrow in aplastic anemia without obviously adverse reaction.
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