3种方法在梅毒实验诊断中的应用价值分析

李果; 高兵

3种方法在梅毒实验诊断中的应用价值分析

李果^,,
高兵

- 华中科技同济医学院附属同济医院检验科(武汉, 430030)

详细信息

通讯作者: 李果,E-mail:tjjyk_lg@126.com

中图分类号: R275.3

收稿日期: 2012-08-31

Values of three methods in detection of syphilis

LI Guo^,,
GAO Bing

- Department of Medical Laboratory, Tongji Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan, 430030, China

More Information

Corresponding author: LI Guo, E-mail: tjjyk_lg@126.com

Received Date: 31 August 2012

摘要

摘要: 目的：探讨分析甲苯胺红不加热血清反应素试验（TRUST）、酶联免疫吸附试验（TP-ELISA）和化学发光法（TP-CMIA）在梅毒实验诊断中的应用价值。方法：收集住院患者输血前检查血清样本8 000例，分别用TRUST、TP-ELISA和TP-CMIA进行梅毒抗体的检测，然后进行对比分析。结果：TRUST检出阳性397例，阳性率为4.96%；TP-ELISA检出阳性290例，阳性率为3.63%；TP-CMIA检出340例，阳性率为4.25%。TP-CMIA和TP-ELISA两者比较差异有统计学意义（P<0.05）。结论：TP-CMIA优于TRUST和TP-ELISA，具有较高的敏感性和特异性，结果观察直观、快速、省时。TP-CMIA能够助于梅毒的准确诊断，适合于常规临床筛查应用，对保障临床输血和手术安全起到积极作用。
- 梅毒 /
- 血清学诊断 /
- 甲苯胺红不加热血清反应素试验 /
- 酶联免疫吸附试验 /
- 化学发光法
Abstract: Objective: To evaluate the clinical significance of Toluidline red unheated serum test (TRUST),Treponema pallidum-enzyme linked immunosorbent assay (TP-ELISA) and Treponema pallidum -chemiluminescence immunoassay(TP-CMIA) in detection of syphilis.Method: We chose 8 000 cases of the patient in hospital.TRUST,TP-ELISA and TP-CMIA were used for each sample.And then we carried out statistical analysis.Result: The samples from 397 patient were positive for the detection with TRUST (positive rate=4.96%);the samples from 290 patient were positive for the detection with ELISA (positive rate=3.63%);and the samples from 340 patient were positive for CMIA (positive rate=4.25%).Significant difference was found between CMIA and ELISA(P<0.05).Conclusion: Compared with TRUST and ELISA,CMIA is more direct,faster and more time-saving.Therefore,CMIA could be used to diagnose the syphilis accurately,and could also be combined to examine syphilis infection,especially before the blood transfusion and surgery.
- syphilis /
- serodiagnosis /
- TRUST /
- TP-ELISA /
- TP-CMIA

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