洛阳地区献血员人类血小板抗原1~10频率分布调查

杨波; 吕运来; 朱丽莉; 吕素梅; 陈善华

doi:10.13201/j.issn.1004-2806-b.2014.06.008

洛阳地区献血员人类血小板抗原1~10频率分布调查

- 洛阳市中心血站(河南洛阳, 471000)

详细信息

通讯作者: 杨波,E-mail:lyxzyb@sina.com

中图分类号: Q343.1

收稿日期: 2013-10-14

Investigation of frequency distribution of human platelet antigen -1to -10 in Luoyang

- Blood Center of Luoyang, Luoyang, 471000, China

More Information

Corresponding author: YANG Bo, E-mail: lyxzyb@sina.com

Received Date: 14 October 2013

摘要

摘要: 目的：研究洛阳地区献血员人类血小板抗原（HPA）1~10基因多态性，为患者提供HPA相配合的血小板。方法：随机收集250例无偿献血员样本，应用聚合酶链式反应-序列特异引物（PCR-SSP）技术检测HPA-1~10基因型及等位基因频率。结果：HPA-1aa、-1ab所占百分比分别为98.80%和1.20%，HPA-2aa、-2ab所占百分比分别为90.80%和9.20%， HPA-4aa、-4ab所占百分比分别为98.00%和2.00%，HPA-5aa、-5ab所占百分比分别为96.40%和3.60%，HPA-aa、-ab、-bb三种基因型在HPA-3和HPA-6中的比例分别为29.20%、46.00%、24.80%和91.60%、6.80%、1.60%，在HPA-7、-8、-9、-10中仅检测到HPA-aa纯合子。结论：洛阳地区HPA-1~HPA-10基因分布与文献报道相似，HPA-3特异性抗体是引起本地区HPA同种免疫性疾病的首要原因。
- 人类血小板抗原 /
- 基因 /
- 血型 /
- PCR-SSP
Abstract: Objective: To study the gene polymorphism of human platelet antigen(HPA) -1 to 10,in order to provide patients with the HPA compatible platelets.Method: A total of 250 samples were collected from the blood donors randomly, and the genotypes and allele frequencies of HPA-1 to 10 were detected by polymerase chain reaction sequence specific primers (PCR-SSP). Result: The percentages of HPA-1aa and-1ab were 98.80% and 1.20% respectively. The percentages of HPA-2aa and-2ab were 90.80% and 9.20% respectively. The percentages of HPA-4aa and-4ab were 98.00% and 2.00% respectively. The percentages of HPA-5aa and-5ab were 96.40% and 3.60% respectively. The proportions of HPA-aa,-ab and-bb in HPA-3 and HPA-6 were 29.20%,46.00%,24.80% and 91.60%,6.80%,1.60% respectively. The HPA-aa homozygous only was detected out in HPA-7,-8,-9,-10.Conclusion: The gene distributions of HPA-1 to HPA-10 was similar to those reported in the literature. The HPA-3 specific antibody was the major cause for HPA autoimmune diseases in Luoyang.
- human platelet antigen /
- gene /
- blood group /
- PCR-SSP

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参考文献(8)

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