Results analysis of testing HBsAg with preliminary test reagent and diagnostic reagent of colloidal gold-labeled method
-
摘要: 目的：分析金标法初筛试剂与诊断试剂检测无偿献血者乙肝表面抗原的阳性率、假阳性率及假阴性率的结果。方法：金标法初筛试纸条检测68 326例，诊断试纸条检测55 637例，乙肝表面抗原初检阴性者与初检阳性者均用ELISA法做复检。结果：初筛试剂检测68 326例的HBsAg阳性率为5.96%、假阳性率5.8%、假阴性率2.84%，诊断试剂检测55 637例的阳性率为2.77%、假阳性率2.6%、假阴性率0.47%。结论：金标法初筛试剂检测无偿献血者乙肝表面抗原的阳性率、假阳性率及假阴性率均高于诊断试剂。Abstract: Objective: To analyze the results of positive rate, false positive rate and false negative rate of testing the voluntary blood donors' HBsAg with preliminary test reagent and diagnostic reagent of colloidal gold-labeled method.Method: 68 326 voluntary blood donors were tested with the preliminary test reagent of colloidal gold-labeled method and 55 637 voluntary blood donors were tested with the diagnostic reagent of colloidal gold-labeled method,all those who were hepatitis B surface antigen negative and who were positive were retested with ELISA.Result: The positive rate, false positive rate and false negative rate of HBsAg among 68 326 voluntary blood donors who were tested with the preliminary test reagent of colloidal gold-labeled method were 5.96%,5.8% and 2.84%, respectively. The positive rate, false positive rate and false negative rate of HBsAg among 55 637 voluntary blood donors who were tested with the diagnostic reagent of colloidal gold-labeled method was 2.77%,2.6% and 0.47%, respectively. Conclusion: The positive rate, false positive rate and false negative rate of the voluntary blood donors' HBsAg which were tested with the preliminary test reagent of colloidal gold-labeled method were higher than those of the diagnostic reagent of colloidal gold-labeled method.
-
Key words:
- colloidal gold-labeled method /
- HBsAg /
- preliminary test reagent /
- diagnostic reagent
-
-
[1] 周继文,戎广亚,杨守纯,等.胶体金免疫层析法检测乙型肝炎病毒表面抗原[J].中华医学检验杂志,1998,21(1):30-32.
[2] 李燕红,王敏,徐燕.金标法在无偿献血工作中的应用分析[J].中国实用医药,2011,6(4):271-271.
-
计量
- 文章访问数: 228
- PDF下载数: 126
- 施引文献: 0
下载: