分子生物学鉴定急性白血病患者ABO血型抗原

陈青; 李平; 陆乐; 肖建宇; 王树亚; 黄成垠; 姚根宏

doi:10.13201/j.issn.1004-2806-b.2015.02.001

分子生物学鉴定急性白血病患者ABO血型抗原

- 1. 江苏省血液中心(南京, 210042);
- 2. 南京大学医学院附属鼓楼医院输血科
基金项目:
江苏省科教兴卫工程医学重点人才(No:RC2011088)

江苏省第4期333高层次人才培养工程,2010年度江苏省卫生国际交流支撑计划项目资助

详细信息

通讯作者: 姚根宏,E-mail:yaogenhong@126.com

中图分类号: R733.7

收稿日期: 2014-06-24

修回日期: 2014-07-29

Molecular genetic analysis of ABO blood group antigens of a patient with acute leukemia

- 1. Jiangsu Province Blood Center, Nanjing, 210042, China;
- 2. Department of Transfusion, the Affiliated Drum Tower Hospital of Nanjing University Medical School

More Information

Corresponding author: YAO Genhong, E-mail: yaogenhong@126.com

Received Date: 24 June 2014

Revised Date: 29 July 2014

摘要

摘要: 目的:应用分子生物学方法鉴定急性白血病患者ABO血型抗原,确定患者ABO血型正反定型不符的原因。方法:选取临床正反定型不一致白血病患者,常规血清学ABO血型正反定型;直接和间接抗人球蛋白试验检测不规则抗体;吸收放散法鉴定弱血型抗原;PCR方法扩增ABO基因的7个外显子及侧翼内含子,扩增产物进行测序及序列分析。结果:患者正定型为AB亚型,反定型为AB型;直抗和间抗为阴性;吸收放散试验证明红细胞上存在弱A和B抗原;ABO基因扩增产物测序结果表明其基因型为ABO*A102/B101。结论:该白血病患者经分子生物学方法确定为正常AB型,其血型抗原减弱由白血病所致。基因分型法可以正确区分血型抗原减弱或亚型。
- 白血病 /
- 血型抗原减弱 /
- 基因分型
Abstract: Objective:To indentify ABO blood group antigen by genotyping, and explore the mechanism of ABO discrepancy in a patient due to acute leukemia. Method:A acute myeloid leukemia patient with ABO typing discrepancy was selected. Routine serological test was carried out to analyze ABO blood group. Both direct and indirect anti-globulin tests were performed to screen irregular antibodies. The presence of the blood group determinants on the red blood cells were determined by adsorption-elution test. Exons 1-7 and adjacent introns of the ABO gene were amplified by PCR and sequenced. Result:ABO forward typing indicated that the patient was AB subgroup. However the reverse typing defined AB. Direct and indirect anti-globulin tests were negative. The RBCs could adsorb anti-A and anti-B, which were able to be eluted subsequently. ABO genotyping was ABO*A102/B101. Conclusion:The leukemia patient with ABO discrepancy by serological test was defined as AB by molecular genetic analysis. The suppressed expressions of A and B antigens on RBCs were due to acute myeloid leukemia. The genotyping could be used to differentiate the subgroup and suppressed expressions of blood group antigens.
- leukemia /
- suppressed expression of blood group antigens /
- genotyping

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参考文献(7)

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