Abstract: Objective:To comprehend the characteristics and infective route of human immunodeficiency virus (HIV) infection in Dongguan voluntary blood donors in order to serve the recruitment of low-risk blood donors. Method:The HIV infectious markers in specimens of Dongguan voluntary blood donors from 2012 to 2013 were detected, and the reactive specimens were sent to Center of Disease Control (CDC) to confirm. The number of positive HIV specimens confirmed was counted. The HIV infectors confirmed were informed and made first follow-up to analyze their epidemiological characteristics, including gender, age, education, occupation and infective route.Result:The infection rate of HIV in Dongguan in 2012 and 2013 was 0.049% and 0.056%, respectively, and the total infection rate was 52.54/100 thousand. The majority of the HIV infectors were male, accounting for 94.0%, and female infectors accounted for 6.0%, the sex ratio of the infection was 15.6:1. The majority ages of the infectors were from 21 to 30, accounting for 45.8%, followed by ages from 31 to 40, accounting for 33.7%, ages from 18 to 20 and from 41 to 50 accounted for 6.0% and 14.5%,respectively,and there was no infector at the ages from 51 to 60.On education, junior high school and below junior high school were the highest proportion in the infectors, accounting for 43.3%, high school and technical secondary school accounted for 41.0%, junior college and above junior college accounted for 15.6%.The main career of the infectors was ordinary worker, accounting for 68.7%, the infectors in commercial and service industries accounted for 16.9%, in students accounted for 1.2%, in medical workers accounted for 3.6%, and in other occupations accounted for 9.6%. All the infective route were sexual transmission, the main infective route was heterosexual transmission, accounting for 55.4%, homosexual transmission between men and men accounted for 31.3%,both having heterosexuality and homosexual behavior between men and men accounted for 13.3%.There was no case through homosexual transmission between women and women. Conclusion:The HIV infectors in Dongguan voluntary blood donation population showed the distribution of multiple ages and multiple occupational groups. The infective route was sexual transmission; the situation was in grim on homosexual transmission between men and men, especially. The inquiry before blood donation and the screening for high risk blood donation population should be specific strengthen to reduce the risk of HIV transmission through blood transfusion.