Rapid detection of methicillin-resistant staphylococcus aureus by Real-time PCR
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摘要: 目的:评价一种直接从标本中快速检测耐甲氧西林金黄色葡萄球菌(MRSA)的实时荧光PCR方法。方法:临床送检标本中筛选出46份金黄色葡萄球菌阳性的标本,进行实时荧光聚合酶链反应(PCR)检测,并与全自动细菌鉴定仪(VITEK-2Compact)/头孢西丁纸片扩散法进行比较,评价实时荧光PCR方法的敏感性及特异性。结果:VITEK-2 Compact分离出金黄色葡萄球菌46株,其中MRSA 12株,与头孢西丁纸片扩散法结果一致。实时荧光PCR法检测出金黄色葡萄球菌46株,其中MRSA 13株,敏感性及特异性分别为100%(95% CI,75.75,100),97.06%(95%CI,85.08,99.48)。2种方法有很好的一致性[Kappa=0.945(95% CI,0.839,1.000)]。结论:实时荧光PCR法是一种能从标本中直接检测MRSA的快速,敏感的方法。
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关键词:
- 耐甲氧西林金黄色葡萄球菌 /
- 实时荧光PCR /
- 医院感染
Abstract: Objective:To evaluate a rapid method for the detection and identification of methicillin-resistant Staphylococcus aureus (MRSA) from clinical specimens. Method:46 Staphylococcus aureus positive clinical samples were detected by real-time PCR. VITEK 2 Compact and cefoxitin disk diffusion method were used as golden standard to evaluate the sensitivity and specificity of the real-time PCR method. Result:VITEK 2 Compact and cefoxitin disk diffusion method identified 46 Staphylococcus aureus strains, of which 12 strains were MRSA. Real-time PCR detected 46 Staphylococcus aureus, including 13 MRSA. The sensitivity and specificity were 100% (95% CI, 75.75, 100)and 97.06% (95% CI, 85.08,99.48), respectively. Real-time PCR method showed very good consistency with the reference method[Kappa=0.945(95% CI,0.839,1.000)]. Conclusion:Real-time PCR method may be a reliable and fast way to detect and identify MRSA directly from the clinical specimen.-
Key words:
- MRSA /
- Real-time PCR /
- Nosocomial infection
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