Genomic characteristics of clinical isolates from HFMD patients in Huangmei city
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摘要: 目的:了解手足口病暴发高峰时段临床患者的咽拭子标本中分离出的人肠道病毒71型(EV71)和柯萨奇病毒A16型(CA16)的基因型,为手足口病的防治提供依据。方法:将手足口病高峰时期收集的咽拭子标本109份进行病毒分离鉴定,对分离到的4株EV71型病毒株的227 bp VP3-VP1基因序列和3株CA16株的736 bp VP1-2A基因序列进行基因测序,并进行系统进化分析。结果:检测到的4株EV71分离毒株均属C4a亚群,3株CA16分离毒株均属于B1b亚群。结论:2013年黄梅县手足口病暴发高峰时段的EV71流行株为C4a亚群,CA16流行株为B1b亚群。Abstract: Objective:To investigate the genetic features of clinical isolates from hand-foot-mouth disease(HFMD) patients in circumjacent Huangmei city.Method:4 strains of Enterovirus 71(EV71) and 3 strains of coxsackievirus A16 (CA16) were isolated from 109 throat swab samples of HFMD cases. Sequencing were done among 227bp VP3-VP1 regions of EV71 and 736bp VP1-2A regions of CA16. Phylogenetic analysis was performed. Result:Phylogenetic analysis showed that 4 EV71 strains belonged to subgenotype C4a, 3 CA16 isolates belonged to subgenotype B1b. Conclusion:EV71 epidemic strains were C4a, and CA16 epidemic strains were B1b in the peak outbreak period of Huangmei city.
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Key words:
- hand-foot-mouth disease /
- Enterovirus 71 /
- Coxsackievirus A16 /
- phylogenetic analysis
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