哮喘患者外周血单个核细胞IL-23/Th-17轴及相关因子mRNA的表达与临床意义

张杨; 梁涛; 许依婷; 王宁; 蔡鹏程; 王晓蓓; 王琳; 胡丽华

doi:10.13201/j.issn.1004-2806-b.2016.04.001

哮喘患者外周血单个核细胞IL-23/Th-17轴及相关因子mRNA的表达与临床意义

- 华中科技大学同济医学院附属协和医院检验科(武汉, 430022)
基金项目:
武汉市科技攻关计划项目(No:2013060602010235)

详细信息

通讯作者: 王琳,E-mail:katelinwang@126.com; 胡丽华, E-mail:lihuahu@hust.edu.cn

中图分类号: R256.12

收稿日期: 2015-09-16

Expression of IL-23/Th-17 axis and other association genes in peripheral blood mononuclear cells from asthma patients

- Department of Clinical Laboratory, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China

More Information

Corresponding authors: WANG Lin ; HU Lihua

Received Date: 16 September 2015

摘要

摘要: 目的: 探讨哮喘患者外周血单个核细胞(PBMCs)中白细胞介素23(IL-23)、白细胞介素17(IL-17)、肿瘤坏死因子α(TNFα)、转化生长因子β(TGFβ)、Foxp3(Foxp3) 基因mRNA的表达及其临床意义。方法: 以实时定量逆转录聚合酶链式反应(RT-PCR)分别检测68例急性发作期哮喘患者及60例健康体检者PBMCs中IL-23、IL-17、TNFα、TGFβ、Foxp3基因mRNA的表达情况,并进一步分析其内在关联性。结果: 与对照组相比,哮喘急性发作期患者PBMCs中的IL-23和TNFα mRNA表达水平显著增高(P<0.05),TGFβ mRNA表达水平显著下降(P<0.05);而IL-17、Foxp3 mRNA表达水平差异无统计学意义(P>0.05);TNFα表达水平分别与IL-17、IL-23呈正相关性,而Foxp3与TGFβ呈正相关性;IL-23 mRNA水平与哮喘患者病程、疾病严重程度和疾病控制程度存在一定关联性。结论: IL-23/Th-17轴在哮喘炎症反应过程中可能发挥潜在的重要作用,IL-23与哮喘的严重程度密切相关,有望作为其治疗监测、预后转归的潜在生物评价指标。
- 哮喘 /
- 白细胞介素23 /
- 白细胞介素17 /
- 肿瘤坏死因子α /
- 转化生长因子β
Abstract: Objective: To investigate the levels of the mRNA expression of IL-23, IL-17, TNFα, TGFβ and Foxp3 gene in peripheral blood monocytes (PBMCs) of asthma patients and their clinical significance. Method: The mRNA expression of IL-23, IL-17, TNFα, TGFβ and Foxp3 in PBMCs of 68 asthma patients and 60 healthy controls were detected using fluorescence quantitative real-time reverse transcription-polymerase chain reaction, and the correlation between them were analyzed. Result: Compared to the normal group, the mRNA expression of IL-23 and TNFα in PBMCs of asthma patients was significantly increased (P<0.05), and TGFβ was significantly decreased (P<0.05), while the expression of IL17 and Foxp3 had no significance between the patients group and the normal group (P>0.05). The mRNA expression of TNFα was positively correlated to IL-17 and IL-23, so as TGFβ to Foxp3. In addition, the expression level of IL-23 mRNA was correlated to the duration of disease, disease severity and disease control in asthma patients. Conclusion: IL-23/Th-17 axis might play an important role in asthma inflammation, and IL-23 may be closely related to the severity of asthma, which is expected as one biomark of its evaluation and treatment.
- asthma /
- IL-23 /
- IL-17 /
- TNFα /
- TGFβ

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参考文献(13)

[1]	Vazquez-Tello A, Halwani R, Hamid Q, et al. Glucocorticoid receptor-beta up-regulation and steroid resistance induction by IL-17 and IL-23 cytokine stimulation in peripheral mononuclear cells[J]. J Clin Immunol,2013,33:466-478.
[2]	Nakajima H, Hirose K. Role of IL-23 and Th17 Cells in Airway Inflammation in Asthma[J]. Immune Netw,2010,10:1-4.
[3]	中华医学会呼吸病学分会哮喘学组.支气管哮喘防治指南(支气管哮喘的定义、诊断、治疗和管理方案)[J].中华结核和呼吸杂志,2008,31(3):177-185.
[4]	Ciprandi G, Cuppari C, Salpietro A, et al. Serum IL-23 in asthmatic children[J]. J Biol Regul Homeost Agents,2012,26:S53-S61.
[5]	Li Y, Sun M, Cheng H, et al. Silencing IL-23 expression by a small hairpin RNA protects against asthma in mice[J]. Exp Mol Med,2011,43:197-204.
[6]	Alyasin S, Karimi MH, Amin R, et al. Interleukin-17 gene expression and serum levels in children with severe asthma[J]. Iran J Immunol,2013,10:177-185.
[7]	Wakashin H, Hirose K, Maezawa Y, et al. IL-23 and Th17 cells enhance Th2-cell-mediated eosinophilic airway inflammation in mice[J]. Am J Respir Crit Care Med,2008,178:1023-1032.
[8]	Song C, Luo L, Lei Z, et al. IL-17-producing alveolar macrophages mediate allergic lung inflammation related to asthma[J]. J Immunol,2008,181:6117-6124.
[9]	Golikova EA, Lopatnikova JA, Kovalevskaya-Kucheryavenko TV, et al. Levels of TNF, TNF autoantibodies and soluble TNF receptors in patients with bronchial asthma[J]. J Asthma,2013,50:705-711.
[10]	Agarwal A, Singh M, Chatterjee BP, et al. Interplay of T helper 17 cells with CD4⁺ CD25 high FOXP3⁺ Tregs in regulation of allergic asthma in pediatric Patients[J]. Int J Pediatr,2014,2014:636238.
[11]	潘珍珍,李羚,郭赟,等.CD4⁺ CD25⁺ Foxp3⁺调节性T细胞与IL-33在儿童哮喘发病机制中的作用[J].中国当代儿科杂志,2014,16(12):1211-1214.
[12]	Shi LZ, Wang R, Huang G, et al. HIF1 alpha-dependent glycolytic pathway orchestrates a metabolic checkpoint for the differentiation of TH17 and Treg cells[J]. J Exp Med, 2011, 208:1367-1376.
[13]	Karagiannidis C, Akdis M, Holopainen P, et al. Glucocorticoids upregulate FOXP3 expression and regulatory T cells in asthma[J]. J Allergy Clin Immunol,2004,114:1425-1433.