19个STR座位基因多态性与胃肿瘤的关联性研究

于来水; 潘凌子; 肖南; 梁晓华; 刘辉

doi:10.13201/j.issn.1004-2806-b.2016.04.002

19个STR座位基因多态性与胃肿瘤的关联性研究

- 1 大连市血液中心(辽宁大连, 116001);
- 2 大连医科大学

详细信息

通讯作者: 梁晓华,E-mail:liangxiaohua@dlbc.org.cn; 刘辉,E-mail:liuhui60@sina.com

中图分类号: R735.2

收稿日期: 2015-07-16

Association between genetic polymorphism of 19 STR loci and gastric cancer

- 1 Dalian Blood Centre, Dalian, 116001, China;
- 2 Dalian Medical University

More Information

Corresponding authors: LIANG Xiaohua ; LIU Hui

Received Date: 16 July 2015

摘要

摘要: 目的: 探讨19个短串联重复序列(STR)座位(D19S433、D5S818、D21S11、D18S51、D6S1043、D3S1358、D13S317、D7S820、D16S539、CSF1PO、Penta D、vWA、D8S1179、TPOX、Penta E、TH01、D12S391、D2S1338、FGA)基因多态性与胃肿瘤的关联。方法: 利用毛细管电泳技术检测38例胃肿瘤患者19个STR座位的基因多态性并进行基因平衡(Hardy-Weinberg)分析,对照组为200例健康个体,通过统计学方法比较两组数据各位点基因频率。结果: 实验组19个STR座位基因分布情况符合遗传规律,STR座位检测结果显示:实验组TH01-7、D5S818-15、D3S1358-18、D7S820-9、Penta E-23、D2S1338-19的频率显著高于对照组(P<0.05),D7S820-12、D12S391-17、D2S1338-20的频率显著低于对照组(P<0.05),Penta E座位纯合子的实际频率显著低于理论频率(P<0.05)。结论: TH01-7、D5S818-15、D3S1358-18、D7S820-9、Penta E-23、D2S1338-19 6个基因座附近可能存在胃肿瘤的易感基因,D7S820-12、D12S391-17、D2S1338-20 3个基因座附近可能存在胃肿瘤的抑制基因,Penta E座位的杂合子表现可能增加患胃肿瘤的风险。
- 胃肿瘤 /
- 短串联重复序列 /
- 多态性
Abstract: Objective: To explore the association between gastric cancer and the genetic polymorphism of 19 STR loci (D19S433, D5S818, D21S11, D18S51, D6S1043, D3S1358, D13S317, D7S820, D16S539, CSF1PO, Penta D, vWA, D8S1179, TPOX, Penta E, TH01, D12S391, D2S1338 and FGA). Method: The capillary electrophoresis was applied to analyze 19 STR loci in 38 patients with gastric cancer and Hardy-Weinberg balance was tested. Two hundred healthy people were collected as control group. The significant differences in frequency of gene and homozygote were analyzed by statistical methods.Result: The gene distributions of 19 STR in experimental group were consistent with hereditary rules. The frequency of TH01-7, D5S818-15, D3S1358-18, D7S820-9, PentaE-23 and D2S1338-19 in the experimental group were significantly higher than those in the control group (P<0.05), while the frequency of D7S820-12, D12S391-17 and D2S1338-20 were obviously lower (P<0.05), and the actual frequency of homozygote at the locus PentaE was obviously lower than the theoretical frequency (P<0.05). Conclusion: There may be predisposing genes of gastric cancer near the six loci of TH01-7, D5S818-15, D3S1358-18, D7S820-9, PentaE-23 and D2S1338-19, while there may be suppressor gene of gastric cancer near the three loci of D7S820-12, D12S391-17 and D2S1338-20, and the expression of heterozygote at the locus Penta E may increase the risk of gastric cancer.
- gastric cancer /
- short tandem repeats /
- polymorphism

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参考文献(8)

[1]	Geiersbach KB, Samowitz WS. Microsatellite instability and colorectal cancer[J].Arch Pathol Lab Med,2011,135:1269-1277.
[2]	Drobnic K, Budowle B. The analysis of three short tandem repeat (STR) loci in the Slovene population by multiplex PCR[J].J Forensic Sci,2000,45:893-895.
[3]	Steinbak A, Malpica A, Slewal A, et al. Biomarkers and microsatellite instability analysis of curettings can predict the behavior of FIGO stage I endometrial endometrioid adenocarcinoma[J].Modern Pathology, 2011,24:1262-1271.
[4]	Zaanan A, Meunier K, Sangar F,et al. Microsatellite instability in colorectal cancer:from molecular oncogenic mechanisms to clinical implications[J].Cell Oncol (Dordr),2011,34:155-176.
[5]	刘争,赵华,罗志永,等.乳腺癌与癌前病变微卫星DNA杂合性缺失研究[J].中国实验诊断学,2011,15(4):592-595.
[6]	Ling XL,Fang DC,Wang RQ, et al. Mitochondrial microsatellite instability in gastric cancer and its precancerous lesions[J]. World J Gastroenterol,2004,10:800-803.
[7]	吕丽琼,刘建勇.乳腺癌微卫星不稳定性及其与临床病理关系的研究[J].广西医科大学学报,2010,27(4):548-552.
[8]	Jemal A, Bray F, Center MM, et al. Global cancer statistics[J].CA Cancer J Clin, 2011,61:69-90.