摘要:
目的:调查浙江地区人群HPA系统遗传多态性特点,探讨临床血小板输注中HPA配型输注的应用效果。方法:采用PCR-SSP方法对400例献血员及54例血小板抗体筛检阳性患者进行血小板抗原HPA1-6,15系统基因分型,对54例患者进行分组血小板输注治疗效果分析。结果:血小板抗原HPA1-6,15系统中HPA-3的b基因频率0.51,系统b基因最高,HPA-15的杂合度0.33,系统杂合度最高,其余系统均以aa纯合子基因分型占绝对优势,超过90%。血小板HPA配型输注组CCI、PPR均明显优于ABO随机输注组。结论:在血小板临床输注治疗中应用HPA配型血小板可防止输注无效的发生率,从而有效提高血小板输注疗效和安全性,值得推广和应用。
Abstract:
Objective:To study the genetic polymorphism of human platelet antigens in zhejiang,understand the trtansufsion effect on platele gene matching group in platele trtansufsion.Method:Platelet antigen genotyping was performed by sequence-specific primer polymerase chain reaction（SSP-PCR）for the HPA1-6,15 systems in 400 platele donors and 54 patients of Zhejiang.The effectiveness of differents platelet transfusion for 54 patients of platelet antibody screening positive.Result:Among HPA1-6,15 systems,HPA-3has the highest b gene frequency,its frequency is 0.51,HPA-15 has the highest heterozygosity,HPA-15 ab 0.33,others systems is mainly aa homozygous genotype,more than 90%.Platelet gene matching group has higher CCI and PPR than the group that receives random platelet group.Conclusion:The effectiveness of patients transfusion gene matching platelet prevent from occurrence of platelet transfusion refractoriness and improve the effective rate of transfusion.
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