Abstract: Objective:To research the iso-immunization reaction of RhD variant.Method:Use microcolumn gel card to detected RhD antigen about 42 940 resident in Jiangxia District from July 2015 to June 2016;The RhD negative samples were confirmed in the preliminary screening RhD negative samples by different RhD reagent.RhD genetic type was performed in the preliminary screening RhD negative samples by PCR-SSP.The weak D in the preliminary screening RhD negative samples were detective by indirect antiglobulin test.The Del in the preliminary screening RhD negative samples were found out by absorption-elution test.Irregular antibody screening and antibody specificity identification were performed in preliminary screening RhD negative samples.Result:In 42 940 resident 179persons were RhD negative weak D 2persons,Del 15 persons were checked out in 179 persons preliminary screening RhD negative samples by serological examination.Weak D 5persons,D-CE（2-9）-D fusion genetic typle 12 persons,Del type 40 persons be inspected by PCR-SSP.D antibody 2persons were checked out in 12person’s RhD-CE（2-9）-D.Conclusion:Different RhD antigenic variant have different immunization.The establishment different transfusion strategies guarantee blood transfusion safety.