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Abstract: To analyze the influence of cold agglutinin on blood typing and cross-matching test,and find out corresponding solution.Micro glass bead column method,tube test,absorption elution test,antiglobulin test of Coombs and cross-matching test with Polybrene were applied to identify specific antibody,and PCR-SSP to confirm the exact blood type together with serologic pattern.Incubation at 37℃,no puzzle with blood typing and cross-matching.Incubation at 37℃,RBC washing at 37℃ and/or cold absorption at 4℃could resolve the discrepancy of blood typing which is mediated by cold agglutinin,also PCR-SSP may further affirm the serologic pattern.
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Key words:
- cold agglutinin /
- blood typing /
- PCR-SSP
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