系统性红斑狼疮患者Th17相关4种细胞因子的研究

严瑾; 胡丽华

doi:10.13201/j.issn.1004-2806-b.2017.12.004

系统性红斑狼疮患者Th17相关4种细胞因子的研究

严瑾,
胡丽华^,

- 华中科技大学同济医学院附属协和医院检验科(武汉, 430022)

详细信息

通讯作者: 胡丽华,E-mail:xhhulh@126.com

中图分类号: R593.24

收稿日期: 2017-08-30

Study of four cytokines related to Th17 cells in patients with systemic lupus erythematosus

YAN Jin,
HU Lihua^,

- Department of Clinical Laboratory, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China

More Information

Corresponding author: HU Lihua, E-mail: xhhulh@126.com

Received Date: 30 August 2017

摘要

摘要: 目的:研究Th17相关的4种细胞因子IL-17,IL-21,IL-22,IL-23在系统性红斑狼疮(SLE)患者与健康人群中血清含量的差别和SLE患者血清中这4种细胞因子的含量与实验室检查指标的关联。方法:收集65例SLE患者和36例健康体检者的血清,用酶联免疫吸附试验(ELISA)检测2组样本血清中Th17细胞相关的4种细胞因子IL-17,IL-21,IL-22,IL-23的含量,将SLE患者与健康人群血清中4种细胞因子的含量进行对比,观察两者之间的差异性;将SLE患者血清中这4种细胞因子的含量与实验室指标进行比较,观察其相关性。采用SPSS19.0进行统计分析。结果:SLE患者血清中IL-17和IL-21的含量显著高于正常对照组(P＜0.01),SLE患者血清中IL-22 和IL-23的水平相对于正常组差异无统计学意义;65例SLE患者血清抗-dsDNA抗体,抗核小体,抗组蛋白阳性组与阴性组比较,阳性组IL-17含量均显著增高(P＜0.05);相反,抗-Sm抗体阳性组与阴性组比较,阳性组IL-17含量显著降低(P＜0.05);65例SLE患者血清中的IL-17,IL-21含量与C₃含量之间均有显著关联(P＜0.05),而这4种细胞因子与IgG含量无关联。结论:SLE患者血清里Th17相关细胞因子IL-17,IL-21 的升高可能促进了疾病的发生与发展;通过对4种细胞因子含量与实验室指标的关联分析,说明IL-17可能促进了SLE患者体内抗-dsDNA抗体,抗核小体,抗组蛋白等的分泌,同时也说明IL-17含量的高低可能对SLE患者疾病的严重程度有一定的提示作用。
- 系统性红斑狼疮 /
- Th17细胞 /
- IL-17 /
- IL-21
Abstract: Objective:To study the serum expression differences of the four kinds of cytokines related to Th17 cells including IL-17, IL-21, IL-22 and IL-23 between the patients with systemic lupus erythematosus (SLE) and the healthy people, and analyze the association between the serum levels of these four cytokines in SLE patient and the laboratory test indicators.Method:The serum of 65 SLE patients and 36 health checkers were collected and the levels of the four cytokines IL-17, IL-21, IL-22 and IL-23 were detected using enzyme linked immunosorbent assay(ELISA). The serum levels of these four cytokines in SLE patients and the healthy group were compared.The serum levels of these four cytokines and other laboratory indicators in SLE patient were compared, and the correlation was analyzed.Statistical analysis was performed using SPSS19.0.Result:The levels of IL-17 and IL-21 in SLE patients were significantly higher than those in the control group (P<0.01). But the levels of IL-22 and IL-23 in SLE patients were not significantly different from those in the control group.Comparing positive group of anti dsDNA antibody, anti-nucleosome antibody and anti-histone antibody in the 65 SLE patients with the negative group, the content of IL-17 in the positive group was significantly higher (P<0.05).On the contrary, comparing the anti-Sm antibody positive group with the negative group, the content of IL-17 in the positive group was significantly reduced (P<0.05). The contents of IL-17, IL-21 and C3 in the 65 SLE patients were significantly correlated (P<0.05),but there was no correlation between these four cytokines and IgG content.Conclusion:The increase of serumIL-17 and IL-21 of SLE patients may contribute to the occurrence and development of the disease.The correlation analysis of four cytokine factors and laboratory indexes showed that IL-17 may promote the secretion of anti-dsDNA antibody, anti-nucleosome antibody and anti-histone antibody in SLE patients, and the level of IL-17 may have a certain effect on the severity of SLE.
- systemic lupus erythematosus /
- Th17 cells /
- IL-17 /
- IL-21

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参考文献(11)

[1]	戴超,李向培.系统性红斑狼疮Th17细胞分子调控机制研究进展[J].安徽医药,2016,20(5):817-820.
[2]	Yang J, Yang X, Zou H,et al.Recovery of the immune balance between Th17 and regulatory T cells as a treatment for systemic lupuserythematosus[J]. Rheumatology,2011,50:1366-72.
[3]	Rother, N.van der Vlag, J.Disturbed T Cell Signaling and Altered Th17 and Regulatory T Cell Subsets in the Pathogenesis of Systemic Lupus Erythematosus[J]. Front Immunol,2015,6:610.
[4]	Júlio Antônio Pereira Araújo a, DaniloMesquitaJr a, Wilson de MeloCruvinel a, et al.Th17 cells and CD4⁺ multifunctional T cells in patients with systemic lupus erythematosus[J]. Rev Bras Reumatol Engl Ed,2016,56:28-36.
[5]	Robak E, Kulczycka-Siennicka L, Gerlicz Z, et al. Correlations between concentrations of interleukin (IL)-17A,IL-17B and IL-17F, and endothelial cells and proangiogenic cytokines in systemic lupus erythematosus patients[J]. Eur Cytokine Network,2013,24:60-68.
[6]	Wang HX, Chu S1, Li J, et al. Increased IL-17 and IL-21 producing TCRαβ⁺CD4^-CD8^- T cells in Chinese systemic lupus erythematosus patients[J]. Lupus, 2014,23:643-54.
[7]	蓝艳,郭静.白细胞介素-21与系统性红斑狼疮之关系研究进展[J].分子诊断与治疗杂志,2015,7(2):127-131.
[8]	Karrich JJ, Jachimowski LC, Nagasawa M, et al. IL-21stimulated human plasmacytoid dendritic cells secretegranzyme B, which impairs their capacity to induce T-cell proliferation[J]. Blood, 2013,121:3103-3111.
[9]	Blom L, Poulsen LK. In vitro Th1 and Th2 cell polarization is severely influenced by the initial ratio of naive and memory CD4⁺T cells[J]. Immunol Methods, 2013,397:55-60.
[10]	鄢海波,赵令,马宁.汉族人系统性红斑狼疮和狼疮肾炎与IL-22受体基因多态性的关系[J].中国现代医学杂志,2017,27(9):44-48.
[11]	Amarilyo G,Lourenco EV,Shi FD,et al.IL-17 promotes murine lupus[J].J Immunol,2014,193:540-543.