Abstract: Objective:To investigate the effect of nitric oxide (NO) donor S-nitrosoglutathione (GSNO) on the metabolism of aphaeresis platelets during storage. Method:Randomly collected six copies of aphaeresis platelet with each about 150-170 ml were mixed and separated into five professional storage bags with each about 30-35 ml. Five groups were consisted of four experimental groups (added with different concentrations of 5mL GSNO respectively and the final concentration were 5,10,100,200 μmol/L) and one control group (added with equal amount of normal saline). The samples were shaked and saved for 7 d at (22±2)℃ and 2 ml platelet were used to count and detection of pH, Lac and MTS at d1, d3, d5 and d7. Result:NO concentration in control group increased slightly with time, while in the experimental groups, high concentration groups (100,200 μmol/L) reached the peak at d3 and low concentration groups (5, 10 μmol/L) peaked at d5. During 7d storage period, the pH value of platelets showed a downward trend and met the requirements at d7. The values of pH, MPV, PDW, pCO₂, pO₂ and cHCO₃ had no differences between treatment and control groups (P=0.07, P=0.47, P=0.59, P=0.15, P=0.64 AND P=0.06). With time, the values of pH, pCO₂ and cHCO₃ in treatment and control groups decreased gradually, while the values of pO₂L, MPV and PDW showed a gradually increasing trend. Lac content decreased with the increasing of GSNO concentration and the content of Lac increased gradually with time. MTS activity of platelets decreased with time in the experimental and controls groups during the preservation period. Platelets with GSNO treatment had no significant differences compared with the control group (P=0.18, P=0.08, P=0.2414, P=0.51). Conclusion:In the preserved process of platelets, NO donor GSNO could inhibit platelet metabolism, reduce lactic acid formation and improve platelet function to some extent.