低频抗-Mur抗体鉴定及血型特征研究

段福才; 曹燕飞; 罗玉华; 祁慧

doi:10.13201/j.issn.1004-2806-b.2018.02.021

低频抗-Mur抗体鉴定及血型特征研究

- 1. 白银市中心血站(甘肃白银, 730900);
- 2. 白银市第二人民医院

详细信息

通讯作者: 段福才,E-mail:906518285@qq.com

中图分类号: R392.11

收稿日期: 2017-07-18

Low frequency of anti-Mur caused by incompatible blood in one case

- 1. Baiyin Blood Center, Baiyin, 730900, China;
- 2. Baiyin Second People's Hospital

More Information

Corresponding author: DUAN Fucai, E-mail: 906518285@qq.com

Received Date: 18 July 2017

摘要

摘要: 目的:通过对1例冰冻保存的抗筛阴性、交叉配血不合的献血者标本进行检测分析,了解低频血型抗体类型和血型特征。方法:采用不同厂家的抗体筛选细胞对冰冻保存标本和新采集标本进行抗体检测和鉴定,并检测抗体效价;采用多种血型血清学方法检测抗-Mur抗体与Mur抗原的红细胞凝集反应特征。结果:该献血者标本与具有Mur抗原的筛选细胞血清凝集反应阳性,与其余筛选细胞反应均为阴性;抗体鉴定结果为IgG抗-Mur抗体;冰冻保存标本和新采集标本的抗体效价均为16;Mur抗原抗体在酶介质中不凝集。结论:导致抗筛阴性、交叉配血不合的不规则抗体为IgG抗-Mur抗体,IgG抗-Mur抗体冰冻保存效价比较稳定且可在人体内较长时间稳定存在,Mur抗原抗体反应符合MN血型抗原抗体血型血清学凝集反应特征。
- Miltenberger血型 /
- 抗-Mur /
- 抗体鉴定 /
- 血型特征
Abstract: Objective:To investigate the types and characteristics of low frequency blood group antibody in 1 case of frozen blood samples with negative screening and incompatible cross matching. Method:The antibodies of frozen specimens and fresh specimens were detected and identified by antibody screening cell of different manufacturers, and antibody titer was detected; red cell agglutination characteristics of anti-Mur antibody and Mur antigen were detected using a variety of serological method.Result:The serum agglutination reaction of specimen of the blood donor with Mur antigen positive cells were positive, and with other screening cells were negative. The antibody identification result was IgG anti -Mur antibody. The antibody titer of frozen preserved specimens and collected samples were 16. Mur antigen and antibody was not agglutinated in the enzyme medium. Conclusion:The irregular antibodies that caused negative screening and incompatible cross matching were IgG anti -Mur antibody, and the anti -Mur antibody of IgG could be stable in human body for a long time. Mur antigen and antibody reaction accorded with serological agglutination reaction characteristics of MN blood group.
- miltenberger blood group /
- anti-Mur /
- antibody identification /
- blood type characteristics

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参考文献(10)

[1]	周娟,刘忠. Miltenberger血型系统的研究进展[J]. 中国输血杂志,2013, 26(12):1294-1296.
[2]	Bakhtary S,Gikas A,Glader B,et al. Anti-Mur as the most likely cause of mild hemolytic disease of the newboro[J]. Transfusion, 2016,56:1182-1184.
[3]	Wei SS, Sun AN, Ding SH, et al. Mur(MNS10) screening with a novel loop-mediated isothermal amplification assay in Zhongshan,China[J]. Transfusion Medicine, 2016,26:215-219.
[4]	Wang HM, Chen YZ, Duan SB, et al. Characterization of a novel microcarrier using magnetized red blood cells with terminal aldehydes[J].Analytical Letters,2016, 49:768-777.
[5]	于洋,王旖,孙晓琳,等. 1994-2013年中国患者人群红细胞同种抗体阳性率及特异性分析[J]. 中国实验血液学杂志,2015, 23(6):1734-1741.
[6]	李翠莹,李运明,黄菲,等. 四川地区汉族人群血型不规则抗体特征分析[J]. 中国实验血液学杂志, 2015, 23(2):533-536.
[7]	周丽莉,董晓峰,王书锋,等. 抗-Mur体抗引起新生儿溶血病1例[J]. 临床输血与检验,,2009, 11(2):1478-1479.
[8]	刘达庄,朱自严,Byme P,等. 低频率抗体抗-Mur引起的溶血性输血反应[J]. 中国输血杂志, 2000, 13(1):8-10.
[9]	李雪群,严凤好.2例无偿献血者抗-mur阳性报道[J]. 国际检验医学杂志, 2015, 36(10):1478-1479.
[10]	王芳,黄霞,毛伟,等. 抗-Mur体抗引起交叉配血不合1例报道[J]. 重庆医学,2013, 42(13):1558-1559.