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摘要: 目的:探讨分析PK7300全自动血型仪在献血者血型筛查中应用的可行性。方法:①用PK7300血型仪反复检测3次100个已经确定血型的标本,看结果是否一致;②用PK7300全自动血型分析仪与目前正在使用的Galileo全自动血型分析仪平行实验,进行ABO血型及RhD血型检测,正反不符、O细胞凝集及RhD阴性标本送往武汉血液中心输血研究室进行结果鉴定。结果:①重复测定3次结果一致;②对24 353名无偿献血者标本进行平行检测,PK7300全自动血型分析仪与Galileo全自动血型仪对ABO血型一次判读正确率分别为99.55%和99.59%;RhD血型一次判读正确率分别为99.93%和99.91%;差异无统计学意义(P>0.05)。结论:PK7300全自动血型仪稳定性好,与Galileo全自动血型分析仪判读结果无统计学差异,结果具有可比性。PK7300全自动血型仪与Galileo全自动血型分析仪比较具有操作更简便,速度更快,试剂用量更少,仪器拍照结果更加清晰直观,人工核对更方便,结果可靠,适合样本量大的血站进行批量化血型检测。
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关键词:
- PK7300全自动血型仪 /
- Galileo全自动血型仪 /
- 血型筛查
Abstract: Objective:To explore the feasibility of applying PK7300 automatic blood grouping analyzer in screening blood donors' blood group.Method: ①100 samples which had been determined were repeatedly detected for 3 times using PK7300 automatic blood grouping analyzer, to see whether the results were consistent. ②ABO blood group and RhD blood group were detected using PK7300 automatic blood grouping analyzer and the Galileo automatic blood grouping analyzer, O cell agglutination and RhD negative specimens were sent to the blood transfusion research department of Wuhan Blood Center to identify.Result:①The results of repeated determination were consistent for 3 times. ②The 24 353 blood donors were detected, and the ABO blood group correct rate of PK7300 automatic blood grouping analyzer and Galileo automatic blood grouping analyzer were 99.55% and 99.59%, respectively; the RhD blood group correct rate were 99.93% and 99.91%, respectively. No statistically significant. Conclusion:PK7300 automatic blood grouping analyzer may have good stability. There was no statistical difference between the results of PK7300 automatic blood grouping analyzer and Galileo automatic blood grouping analyzer, and the results were comparable. Compared with Galileo automatic blood grouping analyzer, PK7300 automatic blood grouping analyzer would have more convenient operation, faster speed, less reagent, more clear instrument picture and intuitive results, more convenient manual checking, which would be reliable and suitable for blood stations with large blood samples. -
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