Abstract: Objective:To investigate the ferritin and hemoglobin status of regular voluntary donors with large number donation,assessment their ferritin and hemoglobin status,and provide evidence for the scientific implement of blood donation.Method:Recruitment from 2006 to 2016 in Qingdao area regular voluntary donors of 45-65 years with large number donation,fasting venous blood samples were drawn for ferritin(FER),red blood cell count(RBC),hemoglobin(HGB),hematocrit(HCT),mean corpuscular volume(MCV).We had statistical analysis according to age and gender groups.45 to 65 years old volunteers were randomly selected from the examination center of a top three hospital in the city as a control.Result:①For male donors of 45-54 years old,FER was significantly lower than that of non-blood donation group(P<0.01),HCT was lower(P<0.05),but RBC,HGB and MCV had no significant difference with control group(P>0.05).②For male donors of 55-65 years old,FER was lower than that of control group(P<0.05),but HGB,RBC,HCT and MCV had no significant difference with control group(P>0.05).③For 45 to 50 years old female donors,FER,HCT,RBC,HGB and MCV had no significant difference with control group(P>0.05).④For 51 to 65 years old female donors,FER was lower than that of control group(P<0.05),HGB and MCV were higher(P<0.05),but HCT and RBC had no significant difference with control group(P>0.05).⑤For male donors,there was a negative correlation between FER,HCT and blood donation(P<0.01),and there was a significant negative correlation between HGB and blood donation(P<0.05).For female there were no negative correlation between FER,HGB,HCT and blood donation(P>0.05).⑥For male donors,higher FER cases were significantly different from control group(P<0.05).But for female,higher FER cases were not different from control group(P>0.05).Conclusion:Male donors'FER,with long-term massive donation of blood over 45 years old,was lower than that of non-blood vonlenteers,but it did not cause a decrease in HGB,RBC and MCV.The decrease in FER was related to blood donation.And higher FER cases were significantly less than control group.Among female blood donors aged 45-50 years there were no reductions in FER,RBC,HGB,HCT and MCV.The FER of female blood donors aged 51-65 years was lower than that of the non-blood donor group.For different gender and age blood donors,different recruitment measures and extension services should be taken.