浙江地区人类血小板抗原基因HPA-1～6,15的多态性分析

曹建平; 赵毓宏; 邓永乐; 程超; 谢珏

doi:10.13201/j.issn.1004-2806-b.2018.10.002

浙江地区人类血小板抗原基因HPA-1～6,15的多态性分析

- 浙江大学医学院附属第一医院输血科(杭州, 310003)

详细信息

通讯作者: 谢珏,E-mail:zyyyxj2011@zju.edu.cn

中图分类号: Q343.1

收稿日期: 2018-07-07

Polymorphism distribution of human platelet antigen gene HPA-1-6,15 in Zhejiang Province

- Department of Blood Transfusion, the First Affiliated Hospital, School of Medicine Zhejiang University, Hangzhou, 310003, China

More Information

Corresponding author: XIE Jue, E-mail: zyyyxj2011@zju.edu.cn

Received Date: 07 July 2018

摘要

摘要: 目的:研究分析浙江地区人类血小板特异性抗原HPA-1~6,15基因分布特点,建立浙江地区HPA基因资料库,为快速寻找基因配合型血小板输注,减少血小板输注无效提供理论基础。方法:采用序列特异性引物-多聚酶链反应(PCR-SSP)方法对345例浙江地区血小板捐献者进行基因分型,比较浙江地区和中国其他地区HPA-1~6,15系统基因型差异。结果:HPA-1~6,15的基因频率分别为HPA-1a 99.57%,HPA-1b 0.43%, HPA-2a 94.93%,HPA-2b 5.07%,HPA-3a 47.54%,HPA-3b 52.46%,HPA-4a 99.86%,HPA-4b 0.14%,HPA-5a 99.57%,HPA-5b 0.43%,HPA-6a 99.71%,HPA-6b 0.29%,HPA-15a 56.23%,HPA-15b 43.77%。经H-W分析,HPA-3,15不符合Hardy-Weinberg遗传定律。与其他地区相比,HPA-2和HPA-5与个别地区有差异。而HPA-3基因频率与多个地区有明显差异,其中b基因频率明显高于a基因频率。结论:浙江地区HPA-1~6,15系统基因分布具有多态性,其中杂合度最高的是HPA-3和HPA-15,在临床血小板输注中这2种血小板特异性抗原容易引起血小板输注无效,需加以重视。其次杂合度较高的是HPA-2,不同的是HPA-3的b基因频率高于a基因。
- 人类血小板特异性抗原 /
- 基因分型 /
- 基因频率
Abstract: Objective:To study and analyze the distribution of human platelet antigen HPA-1-6,15 polymorphism in Zhejiang population and to provide theoretical basis for rapidly finding an antigenic gene matching platelet transfusion and reducing platelet translation refractoriness. Method:Sequence-specific primer polymerase chain reaction(SSP-PCR)was used to analyze platelet antigen HPA-1-6,15 genotypes of 345 platelet donors in Zhejiang and compare with other areas in China. Result:The gene frequency of HPA-1-6,15 were HPA-1a 99.57%,HPA-1b 0.43%,HPA-2a 94.93%,HPA-2b 5.07%,HPA-3a 47.54%,HPA-3b 52.46%,HPA-4a 99.86%,HPA-4b 0.14%,HPA-5a 99.57%,HPA-5b 0.43%,HPA-6a 99.71%,HPA-6b 0.29%,HPA-15a 56.23%,HPA-15b 43.77% respectively.According to H-W analysis,HPA-3,15 do not correspond with Hardy-Weinberg's law of inheritance.Compared with other regions,HPA-2 and HPA-5 differ from individual regions.While the frequency of HPA-3 gene is significantly different from that in many regions,of which the frequency of b gene is significantly higher than that of a gene.Conclusion:The frequencies of HPA-1-6,15 genes are polymorphic.HPA-3 and 15 have the highest heterozygosity followed by HPA-2,which are prone to cause platelet transfusion refractoriness and should be paid more attention to in clinical platelet transfusion.HPA-3 gene polymorphism has its characteristic in Zhejiang province.
- human platelet alloantigen /
- HPA genotyping /
- gene frequency

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