双胎输血综合征患儿A<sub>2</sub>血型2例及其家系基因调查

陈海婧; 张敏; 鲁学良; 孙浩然; 饶神宗

doi:10.13201/j.issn.1004-2806-b.2018.10.010

双胎输血综合征患儿A₂血型2例及其家系基因调查

- 华中科技大学同济医学院附属协和医院(武汉, 430022)

详细信息

通讯作者: 饶神宗,E-mail:1833703604@qq.com

中图分类号: R457.1

收稿日期: 2018-08-09

Identify A₂ blood group in Twin-to-twin transfusion syndrome newborns and pedigree surveys

- Union Hospital Affiliated to Tongji Medical College of Huazhong University of Science and Technology, Wuhan, 430022, China

More Information

Corresponding author: RAO Shenzong, E-mail: 1833703604@qq.com

Received Date: 09 August 2018

摘要

摘要: 目的:分析2例ABO亚型A₂及其家系成员的血型基因。方法:同时应用血清学实验、序列特异性引物聚合酶链反应(PCR-SSP)方法检测患者及其家系成员ABO基因,并进行测序。结果:2例患者血清学结果均为A亚型,PCR-SSP分型结果为A₂₀₅/O₀₂,其母亲血清学结果为O型,PCR-SSP分型结果为O₀₁/O₀₂,其父亲血清学结果为A亚型,PCR-SSP分型结果为A₂₀₅/A₂₀₅,所有家庭成员的PCR-SSP结果与测序结果相符。结论:ABO血型亚型具有遗传基础,可以结合分子生物学方法、血清学方法和家系调查等方法进行最终确认。
- ABO血型 /
- A₂亚型 /
- 家系调查
Abstract: Objective:To test the A₂ blood group in two cases of Twin-to-twin transfusion syndrome newborn and to research the ABO blood group of their family members. Method:The ABO blood group that the patients and their parents were detected by serological experiment,sequence-specific primer polymerase chain reaction(PCR-SSP)and sequencing of gene sequences. Result:The serological results of the patients specimen were typed as A subtype and genotyped as A₂₀₅/O₀₂,the serological results of the mother specimen was typed as O and genotyped as O₀₁/O₀₂,the serological results of the father specimen was typed as A subtype and genotyped as A₂₀₅/O₀₂,and the result of all the family members about sequencing of gene sequences are consistent with PCR-SSP methods.Conclusion:ABO subtype has a genetic basis and can be identified by molecular biological methods combined with serological methods and family surveys.
- ABO blood group /
- A2 subtype /
- family line investigation

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参考文献(8)

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