Expression of PXDN in patients with breast cancer and its clinical value for the prognosis
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摘要: 目的:探讨乳腺癌组织过氧蛋白(PXDN)表达及其对预后的价值。方法:采用定量逆转录聚合酶链反应(qRT-PCR)和免疫组织化学染色检测55例乳腺癌组织和30例良性结节PXDN表达,Kaplan-Meier法绘制不同PXDN表达乳腺癌患者总体生存和无瘤生存曲线,Cox多因素回归模型分析乳腺癌预后的独立危险因素。结果:乳腺癌组织PXDN mRNA相对表达水平和PXDN染色评分明显高于良性结节组织,差异有统计学意义(P<0.05)。乳腺癌组织PXDN表达与年龄、肿瘤大小、TNM分期、人表皮生长因子受体2(Her-2)表达情况无关(P>0.05);PXDN表达与淋巴结转移、雌激素受体(ER)阴性和孕激素受体(PR)阴性有关(均P<0.05)。PXDN高表达组中位无瘤生存时间为53个月,PXDN低表达组中位无瘤生存时间为61个月,差异有统计学意义(χ2=4.185,P=0.041)。PXDN高表达组术后中位总体生存时间为64个月,PXDN低表达组中位总体生存时间为73个月,差异有统计学意义(χ2=4.315,P=0.038)。ER、PR、Her-2是影响乳腺癌无瘤生存的有意义风险因素(均P<0.05);ER、PR、Her-2和PXDN表达是影响乳腺癌总体生存的有意义风险因素(均P<0.05)。结论:乳腺癌组织PXDN表达上调,PXDN高表达是影响乳腺癌患者总体生存时间的独立危险因素。Abstract: Objective:To explore the expression of PXDN in breast cancer and its clinical value for the prognostic prediction. Method:Expression of PXDN in fifty-five breast cancer tissues and thirty benign nodule tissues were detected by using reverse transcription-polymerase chain reaction and immunohistochemical staining,respectively.The over-all survival and disease-free survival curve was drawn by Kaplan-Meier method in different PXDN expression group,independent prognostic factors were analyzed by Cox proportional hazards model. Result:The expression leves and ICH scores of PXDN were increased in the breast cancer tissues when compared to the benign nodule tissues,there was a significant difference between the two groups(P<0.05).PXDN expression was closely correlated with lymph node metastasis,negtive status of ER and PR(P<0.05),but not with age,tumor size,TNM stage,Her-2 status(P>0.05).The median disease-free survival time of the high PXDN group were 53 months,and 61 months in the low PXDN group,there was a significant difference between the two groups(χ2=4.185,P=0.041).The median over-all survival time of the high PXDN group were 64 months,and 73 months in the low PXDN group,there was a significant difference between the two groups(χ2=4.315,P=0.038).Cox multivariate regression analysis showed that ER,PR,Her-2 status was independently associated with disease-free survival of breast cancer,wheras ER,PR,Her-2 status and PXDN expression was independently associated with over-all survival of breast cancer(all P<0.05).Conclusion:PXDN is upregulated in breast cancer.PXDN is an independent prognostic indicator of over-all survival time in patients with breast cancer.
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Key words:
- breast cancer /
- peroxidasin /
- survival curve /
- prognosis
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