Abstract: Objective:To investigate the application of serum HCV-RNA load and anti-HCV concentration detection in the clinical diagnosis of hepatitis C.Method:A total of 100 patients with hepatitis C treated with 2017-04-2018-04 were enrolled in the experimental group.Thirty healthy people in the same period were selected as the control group.The HCV-RNA load was detected by real-time PCR,the anti-HCV concentration was detected by ELISA,and the concentrations of AST,ALT and TBIL in serum were detected by automatic biochemical analyzer,and statistical analysis was performed.Result:Of the 100 specimens,89 were positive for anti-HCV,and the positive rate was 89%.With the increase of HCV-RNA load,the positive rate of anti-HCV increased continuously,the difference was statistically significant(P<0.05).The anti-HCV s/co value of the experimental group was higher than that of the control group,and the difference was statistically significant(P<0.05).The serum AST,ALT and TBIL values of the experimental group were significantly higher than the control group,and the difference was statistically significant(P<0.05).Conclusion:HCV-RNA load combined with anti-HCV and liver function indicators can not only effectively determine the patient's infection and severity,but also monitor clinical treatment.