抗苗勒氏管激素的生物素-链霉亲和素-辣根过氧化物酶酶促化学发光免疫分析方法的建立

薛燕平; 王坤; 李莉华; 任倩; 罗衬银; 张德力; 姚鹏

doi:10.13201/j.issn.1004-2806-b.2019.02.023

抗苗勒氏管激素的生物素-链霉亲和素-辣根过氧化物酶酶促化学发光免疫分析方法的建立

- 1. 黄河三门峡医院检验科(河南三门峡, 472000);
- 2. 黄河三门峡医院妇科;
- 3. 东莞市谢岗医院;
- 4. 东莞市石碣医院
基金项目:
2017年东莞市社会科技发展(一般)项目(No:201750715011315)

详细信息

通讯作者: 姚鹏,E-mail:jykyaopeng@163.com

中图分类号: R459.1

Establishment of biotin-streptavidin-horseradish peroxidase enzymatic chemiluminescence immunoassay for anti-Mullerian hormone

- 1. Department of Clinical Laboratory, Yellow-River Sanmenxia Hospital, Sanmenxia, 472000, China;
- 2. Department of Gynecology, Yellow-River Sanmenxia Hospital;
- 3. Dongguan Xie-gang Hospital;
- 4. Dongguan Shi-jie Hospital

More Information

Corresponding author: YAO Peng, E-mail: jykyaopeng@163.com

摘要

摘要: 目的:抗苗勒氏管激素(AMH)在评价卵巢储备功能方面具有更高的准确性,将其用于青春期、生育期女性的生育指导时具有很好的临床价值。目前对于AMH的检测,临床上常采用的是酶联免疫分析法,但存在灵敏度低、检测范围窄等缺点,而影响AMH实际水平的测定,使得对病情的预估不准确对疾病的治疗产生影响。因此,建立一种低成本、高敏感度的检测AMH水平的方法尤为重要。方法:利用生物素-亲和素放大系统,将一组抗AMH的第一抗体包被微孔板,同时用生物素标记第二抗体,与第一抗体-抗原复合物结合之后,再与生物素-链霉亲和素-辣根过氧化物酶结合物结合,加入发光底物,建立AMH酶促化学发光免疫分析方法,同时对这一体系的线性、灵敏度、特异度、稳定性、回收率等参数进行检测,并与常规ELISA法进行相比检测AMH浓度。结果:线性检测范围为(0.42~50.00)U/ml,该体系稳定性好,灵敏度0.42 U/ml,批内差异小于10.0%,批间差异小于10.0%,与常规ELISA对比差异有统计学意义。结论:AMH的生物素-链霉亲和素-辣根过氧化物酶酶促化学发光免疫分析易于操作、灵敏度高、价格低廉,适用于临床检测。
- 抗苗勒氏管激素 /
- 生物素 /
- 链霉亲和素 /
- 辣根过氧化物酶 /
- 酶促化学发光免疫分析方法
Abstract: Objective:Anti-Mullerian hormone(AMH)has a higher accuracy in evaluating ovarian reserve function,and it is of great clinical value in the reproductive guidance of female during puberty and growing period.At present,the enzyme-linked immunoassay is commonly used in the detection of AMH,but there are some disadvantages,such as low sensitivity and narrow detection range,which affect the actual level of AMH,which makes the prediction of the condition inaccurate to the treatment of the disease.Therefore,it is very important to establish a low cost and high sensitivity method to detect AMH level.Method:Using the Biotin-affinity amplification System,a group of anti-AMH first antibody packages were coated with microporous plates,a method of AMH enzyme-induced chemiluminescence immunoassay was established by combining the biotin-streptavidin-horseradish peroxidase binding with biotin-labeled second antibody and the first antibody-antigen complex,and adding the luminescent substrate,the parameters such as linearity,sensitivity,specificity,stability and recovery rate of the system were detected,and compared with the conventional Elisa method to detect AMH concentration.Result:The linear detection range is(0.42-50.00)U/ml,the system has good stability,sensitivity 0.42 U/ml,the difference between batches is less than 10%,the difference between batches is less than 10%,and the difference is statistically significant compared with the conventional ELISA.Conclusion:AMH biotin-streptavidin-horseradish peroxidase enzymatic chemiluminescence immunoassay is easy to operate,high sensitivity and low price,and is suitable for clinical detection.
- anti-mullerian hormone /
- biotin /
- streptomyces affinity /
- horseradish peroxidase /
- enzymatic chemiluminescence immunoassay

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