联合3种不同诊断指标对新生儿败血症诊断价值分析

王婵; 朱武; 陈垣; 汪学耀

doi:10.13201/j.issn.1004-2806-b.2019.04.005

联合3种不同诊断指标对新生儿败血症诊断价值分析

- 1. 安康市中医医院检验科(陕西安康, 725000);
- 2. 安康市中医医院儿科;
- 3. 汉滨区第三人民医院检验科

详细信息

通讯作者: 汪学耀,E-mail:wxylgqzz@163.com

中图分类号: R722.13

收稿日期: 2018-11-08

Diagnostic value of combined detection of three different diagnostic indexes for neonatal septicemia

- 1. Department of Clinical Laboratory, Ankang Traditional Chinese Medicine Hospital, Ankang, 725000, China;
- 2. Department of Pediatrics, Ankang Traditional Chinese Medicine Hospital;
- 3. Department of Clinical Laboratory, Third People's Hospital of Hanbin District

More Information

Corresponding author: WANG Xueyao, E-mail: wxylgqzz@163.com

Received Date: 08 November 2018

摘要

摘要: 目的：分析联合检测CD14⁺/CD16⁺、肿瘤坏死因子-α（TNF-α）及降钙素原（PCT）3种不同诊断指标对新生儿败血症的诊断价值。方法：将2016-01-2018-03收治的66例新生儿败血症患儿作为病例组，另同期选取30例健康新生儿为对照组。采集2组新生儿血清标本，CD14⁺/CD16⁺的检测采用流式细胞技术，TNF-α的检测采用酶联免疫吸附法，PCT的检测采用免疫发光法，并通过绘制受试者工作特征（ROC）曲线分析单独或联合检测CD14⁺/CD16⁺、TNF-α及PCT对新生儿败血症的诊断效能，计算曲线下面积、敏感度及特异度。结果：病例组患儿血清CD14⁺/CD16⁺、TNF-α及PCT水平较对照组均显著升高（均P<0.01）。ROC曲线结果显示，CD14⁺/CD16⁺以8.48%、TNF-α以7.52 pg/ml、PCT以1.02 ng/ml为诊断阈值时，三者联合检测对新生儿败血症的诊断效能最高，其曲线下面积为0.85，灵敏度和特异度分别为93.94%（62/66）、80.00%（24/30）；CD14⁺/CD16⁺、TNF-α及PCT单独检测对新生儿败血症的诊断效能较低，其ROC曲线下面积分别为0.65、0.69、0.76，灵敏度分别为69.70%（46/66）、77.27%（51/66）、80.30%（53/66），特异度分别为66.67%（20/30）、73.33%（22/30）、70.00%（21/30）。三者在诊断阈值时联合检测与新生儿生后实验诊断结果基本一致，并无显著性差异（χ²=0.10，P=0.75）。结论：联合检测CD14⁺/CD16⁺、TNF-α及PCT对新生儿败血症具有良好的诊断价值，可视为临床重要的诊断方法。
- 新生儿败血症 /
- CD14⁺/CD16⁺ /
- 肿瘤坏死因子-α /
- 降钙素原 /
- 诊断价值
Abstract: Objective: To analyze the diagnostic value of combined detection of CD14⁺/CD16⁺, tumor necrosis factor-alpha(TNF-α) and procalcitonin(PCT) in neonatal sepsis.Method: A total of 66 cases of neonatal sepsis in our hospital from January 2016 to March 2018 were selected as case group and 30 healthy neonates as control group.Serum samples were collected from two groups of neonates.CD14⁺/CD16⁺ was detected by flow cytometry, TNF-α was detected by enzyme-linked immunosorbent assay, and PCT was detected by immunoluminescence assay.The area, sensitivity and specificity of the receiver operating characteristic(ROC) curve were calculated.Result: The serum levels of CD14⁺/CD16⁺, TNF-α and PCT in the case group were significantly higher than those in the control group(P<0.01).ROC curve showed that when CD14⁺/CD16⁺ was 8.48%, TNF-α was 7.52 pg/ml, and PCT was 1.02 ng/ml, the combined detection of CD14⁺/CD16⁺ and TNF-α was the most effective in the diagnosis of neonatal sepsis.The area under the curve was 0.85, and the sensitivity and specificity were 93.94%(62/66) and 80.00%(24/30), respectively.The diagnostic efficiency of CD14⁺/CD16⁺, TNF-α and PCT alone was lower in neonatal septicemia.The ROC curves were 0.65, 0.69 and 0.76, with sensitivity of 69.70%(46/66), 77.27%(51/66) and 80.30%(53/66), specificity of 66.67%(20/30), 73.33%(22/30) and 70.00%(21/30), respectively.The results of combined detection of the diagnostic thresholds were basically consistent with those of postnatal infants, and there was no significant difference(χ²=0.10, P=0.75).Conclusion: Combined detection of CD14⁺/CD16⁺, TNF-α and PCT might have a good diagnostic value for neonatal sepsis, which can be regarded as an important clinical diagnostic method.
- neonatal sepsis /
- CD14⁺/CD16⁺ /
- tumor necrosis factor-alpha /
- procalcitonin /
- diagnostic value

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