Abstract: Objective: To analyze the population structure of Hepatitis C reaction among voluntary blood donors in Lanzhou Blood center of PLA from 2011 to 2016, and provide reference countermeasures for recruiting low-risk blood donors.Method: The Hepatitis C antibody of 55 120 blood donors was determined by ELISA using primary and re-examination reagents, and the characteristics of the donor population with positive results were statistically analyzed.Result: The positive results of primary and retest reagents were slightly different, resulting in a difference in the results of the two reagents and the difference in the sensitivity of the two indicators.Of the 55 120 donors, a total of 519 cases(0.942%) were positive reaction.The difference of positive detection rate of Hepatitis C in differentgender, ethnicity, blood donation category, education level, date of birth and number of blood donations was statistically significant(P<0.05), and there was no statistically significant difference in household registration and marriage(P>0.05).Conclusion: The characteristics of positive Hepatitis C donors were male, born in 1960 to 1979, ethnic minorities, high school and below, participated in mutual aid and blood donation and one time of participation.During voluntary blood donor recruitment, we should pay great attention to the characteristics of such blood donors to the greatest extent to ensure blood safety.