Abstract: Objective: To compare the difference of two chemiluminescence analyzers in detecting specific antibodies to syphilis, using Treponema pallidum immunoblotting(TP-WB) as the gold standard.Method: Eighty cases of positive syphilis positive samples detected by Chemimetric and 80 positive cases of syphilis positive samples detected by Abbott were selected respectively.Syphilis-specific antibodies of the 160 samples were detected using Kemei CLIA and Abbott CMIA chemiluminometer, and retested by WB method.Result: The positive predictive value, negative predictive value and coincidence rate of Kemei CLIA were 90.07%, 88.89% and 90.00%, respectively.The positive predictive value, negative predictive value and coincidence rate of Abbott CMIA were 93.19%, 100.00% and 93.75%, respectively.There was no significant difference between the two types of chemiluminometer(χ²=0.950 P=0.330, χ²=1.513 P=0.219, and χ²=1.507 P=0.220).When Kemei S/CO>9.01 the positive predictive value was 100%, when Abbott S/CO>6.01 the positive predictive value was 100%, and 108 samples had the P47 feature band, accounting for 78.83%, which was the highest frequency occurred in all feature bands.Conclusion: Kemei CLIA chemiluminometer and Abbott CMIA chemiluminometer might have comparatively high coincidence rate with WB method when detecting syphilis.The test results would be true positive S/CO values when the Abbott S/CO value is more than 6.01, and the Kemei S/CO ratio is more than 9.01.In clinical work, Kemei CLIA can be used as an effective supplement to Abbott CMIA because of its lower price.