洛阳地区无偿献血人群中核酸检测及分析

陈善华; 朱丽莉; 吕素梅; 吕冬冬

doi:10.13201/j.issn.1004-2806-b.2019.04.016

洛阳地区无偿献血人群中核酸检测及分析

- 洛阳市中心血站检验科(河南洛阳, 471000)

详细信息

通讯作者: 陈善华,E-mail:1521728943@qq.com

中图分类号: R512.6;R512.91

收稿日期: 2018-12-26

Analysis of nucleic acid detection and follow-up of voluntaryblood donors in Luoyang area

- Luoyang Blood Center, Luoyang, 471000, China

More Information

Corresponding author: CHEN Shanhua, E-mail: 1521728943@qq.com

Received Date: 26 December 2018

摘要

摘要: 目的：了解洛阳地区无偿献血人群中核酸检测及追踪情况。方法：对2012-03-11-2017-12-31参与无偿献血的献血者标本进行2次酶联免疫法和1次核酸检测，并对ELISA检测无反应性、核酸检测有反应性的献血者进行跟踪检测，定期采集其血样进行血液筛查和补充试验，以便确定是否为"窗口期"。结果：163 436份无偿献血者血液标本中检出酶联免疫法无反应性核酸联检有反应性标本310份，进一步对这310份标本进行病毒种类的鉴别，最后鉴别出乙型肝炎病毒（HBV DNA）阳性119份，人类免疫缺陷病毒（HIV RNA）阳性1份，丙型肝炎病毒（HCV RNA）阳性0份。最终随访到19例献血者（鉴别结果为HBV DNA阳性的献血者7例，经确认有5例OBI，2例HBV "窗口期" ；鉴别结果为阴性的献血者11例，经确认有1例HBV "窗口期" ；鉴别结果为HIV RNA阳性的献血者1例确认结果是HIV "窗口期" ）。结论：核酸检测确实能够最大限度地减少方法学上引起的血液安全风险，最大程度地提升血液安全，这对保证用血者健康具有重要意义。
- 核酸检测 /
- 乙型肝炎病毒 /
- 丙型肝炎病毒 /
- 人类免疫缺陷病毒
Abstract: Objective::To understand the nucleic acid detection and follow-up of voluntary blood donors in Luoyang area.Method: From March 11, 2012 to December 31, 2017, two times of Enzyme-linked immunosorbent assay(ELISA) and one nucleic acid test were performed on blood specimens from voluntary blood donors who came to our blood station.The blood donors whose blood specimens did not exhibit reactivity in ELISA, but exhibited reactivity in nucleic acid detection were followed up;and their blood specimens were collected regularly for blood screening and supplementary tests in order to determine whether they were in a "window period".Result: Totally 310 of 163 436 blood specimens from donors were found to exhibit no reactivity in ELISA, but exhibit reactivity in nucleic acid detection.The 310 specimens were further identified for virus types, and finally 119 HBV DNA positive, 1 HIV RNA positive and 0 HCV RNA positive were identified.Finally, 19 blood donors in them were followed up successfully.In 7 blood donors with positive HBV DNA, 5 cases of occult HBV infection(OBI) and 2 cases of HBV " window period" were confirmed.In 11 blood donors with negative HBV DNA, 1 case of HBV " window period" was confirmed.The only donor with positive HIV RNA was confirmed to be HIV "window period".Conclusion: Nucleic acid detection can minimize the risk of blood caused by methodology and improve blood safety to the greatest extent, which is of great significance for ensuring the health of blood recipients.
- nucleic acid detection /
- HBV DNA /
- HCV RNA /
- HIV RNA

HTML全文

参考文献(10)

[1]	Allainl JP, Candotti D.Diagnostic algorithun for HBV safe transfusion[J].Blood Transfus, 2009, 7:174-182.
[2]	张静, 王素玲, 赵莉华, 等.2010-2015年石家庄市无偿献血人群HIV感染率及血液筛查后残余风险评估[J].临床血液学杂志, 2016, 29(10):812-814.
[3]	Stramer SL, Wend U, Candotti D, et al.Nucleic acid testing to detect HBV infection in blood donors[J].N Eng J Med, 2011, 364:236-247.
[4]	朱守兵, 刘祎, 傅立强.绍兴地区无偿献血者血液核酸检测效果评估[J].临床血液学杂志, 2016, 29(10):831-832.
[5]	夏传友, 刘志泉, 陈永飞, 等.佛山市顺德区无偿献血者核酸检测情况分析[J].中国输血杂志, 2017, 30(8):935-937.
[6]	周怡, 蒋靓, 王凯, 等.HBsAg阴性HBV DNA阳性无偿献血者的血清学模式及其相关特征研究[J].临床血液学杂志, 2017, 30(2):86-89.
[7]	Allain JP, Candotti D.ISBT HBV Safety Collaborative Group.Hepatitis B virus in transfusion medicine:still a problem[J].Biologicals, 2012, 40:180-186.
[8]	Lelie N, Bruhn R, Busch M, et al.Detection of different catego-ries of hepatitis B virus(HBV)infection in a multi-regional study comparing the clinical sensitivity of hepatitis B surface antigen and HBV-DNA testing[J].Transfusion, 2017, 57:24-35.
[9]	何成涛, 傅强, 黄敏, 等.血站HIV感染标志物检测方法比较及模型探讨[J].临床血液学杂志, 2017, 30(2):144-146.
[10]	Pillonel J, Laperche S, Saura C, et al.Trends in residual risk of transfusion-t-ransmitted viral inofctions in France between 1992 and 2000[J].Transfusion, 2009, 2:980-982.