苏州市RhD阴性献血者血型抗原及表型分布研究

何红红; 汤龙海; 陆荣; 潘志荣; 金一鸣; 周晓华; 王明元

doi:10.13201/j.issn.1004-2806-b.2019.12.011

苏州市RhD阴性献血者血型抗原及表型分布研究

- 苏州市中心血站检验科(江苏苏州, 215006)
基金项目:
苏州市科技计划项目合同(No:SYS201664)

2017年度苏州市第十九批产业技术创新专项(民生科技)(No:SS201750)

详细信息

通讯作者: 王明元,E-mail:mingyuan98@aliyun.com

中图分类号: R457.1

收稿日期: 2019-04-02

Distribution of phenotype and blood type antigen in RhD negative blood donors in Suzhou

- Department of Clinical Laboratory, Suzhou Blood Center, Suzhou, 215006, China

More Information

Corresponding author: WANG Mingyuan, E-mail: mingyuan98@aliyun.com

Received Date: 02 April 2019

摘要

摘要: 目的:分析苏州地区Rh阴性血型抗原分布特点及表现型规律,为临床输血提供理论依据。方法:选取苏州市中心血站2015—2018年无偿献血者344 367例,运用盐水试管法初筛为RhD阴性样本869例,再用抗人球蛋白微柱凝胶卡式法对其确认,用盐水试管法进行C、c、E、e抗原检测以确定表型。结果:初筛869例RhD阴性标本中有844例标本(97.12%,844/869)确证阴性,占本地区献血者0.25%(844/344 367);D变异型25例(2.88%,25/869),占本地区献血者0.007%(25/344 367),其中以ccEe频率最高(40%,10/25);RhD阴性样本中以ccee、Ccee表现型居多,占比56.40%(476/844)、32.70%(276/844);e、c的抗原频率最高,分别为98.93%(835/844)、95.85%(809/844);RhD阴性献血者主要为A、O、B血型,AB型最少。结论:苏州地区RhD阴性献血者主要表现型为ccee、Ccee,D变异型中ccEe频率最高。对RhD阴性血液样本的定型及对D变异型的筛查,有助于保障本地区临床输血安全,为苏州市稀有血型库的建立提供参考依据。
- RhD阴性 /
- D变异型 /
- RhD表现型 /
- 稀有血型库
Abstract: Objective:To investigate the characteristic and distribution of phenotype and blood type antigen in RhD negative blood donors in Suzhou,and provide a theoretical basis for clinical blood transfusion.Method:869 blood samples of RhD negative were screened among the 344 367 blood donors from 2015 to 2018 in Suzhou blood center,and confirmed by the anti-human globulin microcolumn gel card and serologic blood tests for phenotypes.Result:869 RhD negative specimens were confirmed to be RhD negative 844(97.12%,844/869),accounting for 0.25%(844/344 367)of the donors in the region;D variant phenotypes 25(2.88%,25/869),accounting for 0.007%(25/344 367),ccEe(40%,10/25)had the highest frequency.The RhD negative were mostly ccee and Ccee phenotypes,accounting for 56.40%(476/844) and 32.70%(276/844),and the e and c antigen occupied higher antigen frequencies,98.93%(835/844)and 95.85%(809/844)respectively.RhD negative phenotypes were mostly A,O,B types.Conclusion:The main phenotypes of RhD negative blood donors in Suzhou were ccee and Ccee,and the ccEe frequency was the highest in D variant.The characteristic of RhD negative blood samples and the screening of D variant would help to ensure the safety of clinical blood transfusion and provide reference for the establishment of rare blood bank in Suzhou.
- RhD negative /
- D variant /
- RhD negative phenotype /
- rare blood bank

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参考文献(5)

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