Drug resistance phenotype and nosocomial infection control of multiple drug resistance Klebsiella pneumoniae
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摘要: 目的:研究临床分离的多重耐药肺炎克雷伯菌(MDRKP)的耐药表型及同源性,为有效预防及控制医院感染提供依据。方法:收集44株MDRKP临床分离株,用MIC法进行药敏试验;采用脉冲场凝胶电泳(PFGE)技术对菌株进行分子分型,建立医院PFGE指纹图谱库,运用Bionumeries生物分析软件对菌株之间的亲缘关系及同源性进行分析。结果:44株MDRKP对16种临床常见抗菌药物呈广泛耐药,其中对氨苄西林、哌啦西林/他唑巴坦、头孢唑林他和喹诺酮类抗生素100%耐药,以呼吸科和ICU占比最高(分别占27.2%和25.0%)。PFGE分子分型共分为16个谱型,优势型分别为K12和K13型,菌株间相似性系数达83.0%以上。结论:同病区菌株呈高度同源性分布,且可能存在院内的同源暴发,应加强细菌耐药性监测和医院感染的监控。
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关键词:
- 多重耐药肺炎克雷伯菌 /
- 脉冲场凝胶电泳 /
- 医院感染
Abstract: Objective:To investigate the drug-resistant phenotype and homology of multidrug-resistant Klebsiella pneumoniae(MDRKP)isolated from hospital as so to provide reference for the effective control of nosocomial infections.Method:A total of 44 clinical isolates of MDRKP were collected,and the drug sensitivity test was carried out by MIC method.The molecular typing of the strains was carried out by pulsed field gel electrophoresis(PFGE),and the PFGE fingerprint Library of the hospital was established.The Bionumeries genetic analysis software was used to analyze the phylogenetic relationship and homology between the strains.Result:44 strains of MDRKP were extensively resistant to 16 common clinical antibiotics.PFGE molecular typing was divided into 16 spectrum types,the dominant types were K12 and K13,and the similarity coefficient between strains was more than 83.0%.Conclusion:The strains in the same ward are highly homologous,and there may be homologous outbreaks in the hospital.The surveillance of bacterial resistance and nosocomial infection should be strengthened. -
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