自然杀伤细胞免疫球蛋白样受体及其配体HLA-C与HCV感染的相关性研究

邵雷; 马玲; 史丽莉; 林红

doi:10.13201/j.issn.1004-2806-b.2020.06.001

自然杀伤细胞免疫球蛋白样受体及其配体HLA-C与HCV感染的相关性研究

- 江苏省血液中心输血研究室(南京, 210042)
基金项目:
江苏省第五期“333高层次人才培养工程”项目(No:2016-Ⅲ-3345)

2016年高层次卫生人才“六个一工程”项目(No:LGY2016045)

详细信息

通讯作者: 林红,E-mail:linhong712003@sina.com

中图分类号: R512.6

收稿日期: 2019-11-25

Frequency of killer cell immunoglobulin-like receptors and HLA-C in Jiangsu blood donors with HCV infection

- Jiangsu Province Blood Center, Nanjing, 210042, China

More Information

Corresponding author: LIN Hong, E-mail: linhong712003@sina.com

Received Date: 25 November 2019

摘要

摘要: 目的:自然杀伤细胞免疫球蛋白样受体(KIRs)是自然杀伤(NK)细胞上介导天然免疫的主要受体,同时KIRs也在T细胞上表达,与获得性免疫相关。本研究在对KIRs及其配体HLA-C进行分型的基础上,探讨KIRs、HLA-Cw的基因分型及其联合体与HCV感染的相关性。方法:采用序列特异性引物聚合酶链反应(PCR-SSP)法,对江苏献血人群中164例抗-HCV酶联免疫吸附试验(ELISA)双试剂反应性献血者(抗-HCV阳性组)和241例合格献血者(对照组)的KIRs和HLA-C的基因型进行检测和分析。结果:HLA-Cw的基因分型在抗-HCV阳性组和对照组中的分布无统计学意义;KIRs中与HLA-C配对的KIR基因分型仅有KIR2DS2在2组中的表达频率有差异(对照组21.2% vs抗-HCV阳性组37.8%),差异有统计学意义(P＜0.01);HLA-C1C1与KIR2DS2的配对组合在2组中差异有统计学意义(22.1% vs 42.0%,P＜0.05)。结论:江苏献血者人群中KIR2DS2可能是HCV感染的相关基因;HLAC1C1/KIR2DS2的配对组合可能激活了NK细胞和其他免疫细胞的功能,抑制病毒复制或促进HCV清除的功能,有待进一步实验证实。
- HCV感染 /
- HLA-C基因 /
- KIRs基因 /
- 献血者
Abstract: Objective:Killer cell immunoglobulin-like receptors（KIRs）are the primary receptors of NK cells that mediate innate immunity.KIRs are also involved in acquired immunity,because some KIRs are expressed on the surface of certain subsets of T cells.In this study,the frequency of KIR genes,HLA-C allotypes,and combinations of KIR genes with their HLA-C ligands were analyzed in HCV infection blood donors. Method:HLA-C and KIR genes in two different groups of the Chinese Jiangsu population:241 eligible blood donors（the control group）and 164 double ELISA of anti-HCV reaction blood donors（anti-HCV positive group）were evaluated by PCR-SSP. Result:The distribution of HLA-Cw genotyping in the anti-HCV positive group and the control group was not statistically significant（P＞0.05）.The frequency of KIR2 DS2 in anti-HCV positive group（37.8%）was higher than that in the control group（21.2%）（P＜0.01）.In different combinations of KIR genes with their HLA-C ligands,HLA-C1 C1/KIR2 DS2 had statistical significance between the two groups（P＜0.05）.Conclusion:This study can Among the blood donors in Jiangsu Province, KIR2 DS2 might be associated with HCV infection and the combination of HLA-C1 C1/KIR2 DS2 may be activate the function of NK cells and other immunity cells.
- hepatitis C virus infection /
- HLA-C gene /
- KIRs /
- blood donors

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