血浆微小核糖核酸-155表达与冠状动脉慢血流的相关性研究

杨朋康; 董昕

doi:10.13201/j.issn.1004-2806-b.2020.06.005

血浆微小核糖核酸-155表达与冠状动脉慢血流的相关性研究

杨朋康¹,
董昕^2, ,

- 1. 西安市第一医院心内科(西安, 710002);
- 2. 西安市第四医院心血管内科
基金项目:
陕西省重点研发计划(No:2017SF-039)

详细信息

通讯作者: 董昕,E-mail:3200737205@qq.com

收稿日期: 2019-09-17

Study on correlation between expression of microRNA-155 in plasma and phenomenon of slow coronary flow

YANG Pengkang¹,
DONG Xin^2, ,

- 1. Department of Cardiology, Xi'an First Hospital, Xi'an, 710002, China;
- 2. Department of Internal Medicine-Cardiovascular, Xi'an Fourth Hospital

More Information

Corresponding author: DONG Xin, E-mail: 3200737205@qq.com

Received Date: 17 September 2019

摘要

摘要: 目的:探讨血浆微小核糖核酸-155(miR-155)表达水平与冠状动脉慢血流(CSF)现象的关系。方法:连续性选择2017-01—2019-06行冠状动脉造影检查符合CSF诊断标准的患者62例作为病例组,另随机选择同期接受冠状动脉造影检查无明显血管狭窄且冠脉血流速度正常的患者60例为对照组。采用校正的心肌梗死溶栓血流帧数(cTFC)法检测并计算平均心肌梗死溶栓治疗(TIMI)帧数值(mTFC),采用实时荧光定量PCR(RT-qPCR)法检测血浆miR-155表达水平。结果:病例组血浆miR-155相对表达水平和mTFC均显著高于对照组,差异有统计学意义(Z=3.219,P=0.001;Z=3.219,P=0.001)。Pearson相关性分析结果示:血浆miR-155水平与mTFC值呈正相关关系(r=0.322,P=0.000)。多因素Logistic回归分析结果示:血浆miR-155相对表达水平是CSF现象的独立危险因素(OR=1.597,95%CI 1.109~2.301,P=0.005)。ROC曲线分析结果示:血浆miR-155预测CSF的曲线下面积为0.669(0.574,0.764),cut-off值为24.19时,对应的灵敏度和特异度分别为53.2%和75.0%。结论:血浆miR-155表达水平与CSF现象密切相关,miR-155可作为CSF现象的早期预测因子。
- 微小核糖核酸-155 /
- 冠状动脉慢血流 /
- 相关性研究
Abstract: Objective:To investigate the relationship between miR-155 in plasma and coronary slow flow（CSF）phenomenon. Method:A total of 62 CSF patients and 60 controls who underwent coronary angiography（CAG）in our hospital from January 2017 to June 2019 were selected into this study.Correct thrombolysis in myocardial infarction frame count（cTFC）was used to calculate the mean thrombolysis in myocardial infarction flame counts（mTFC）.Real-time quantitative PCR（RT-qPCR）was used to determine the expression of miR-155 in plasma. Result:The plasma miR-155 level and mTFC in case group were both significantly higher than those in control group（Z=3.219,P=0.001;Z=3.219,P=0.001）.Pearson correlation analysis showed that there was a positive correlation between plasma miR-155 and mTFC（r=0.322,P=0.000）.Multiple Logistics regression analysis showed that plasma miR-155 was independent risk factor for CSF（OR=1.597,95%CI 1.109-2.301,P=0.005）.The area under the ROC curve of miR-155 predicting for CSF was 0.669（0.574,0.764）.When the cut-off value was set at 24.19,the Youden index reached the maximum with a sensitivity of 0.532 and a specificity of 0.750.Conclusion:The expression level of miR-155 in plasma is significantly correlated with CSF,and miR-155 could be used as a predictor of CSF.
- miR-155 /
- coronary slow flow /
- correlation study

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参考文献(17)

[1]	Yılmaz M,Korkmaz H,Bilen MN,et al.Could neutrophil/lymphocyte ratio be an indicator of coronary artery disease,coronary artery ectasia and coronary slow flow?[J].J Int Med Res,2016,44:1443-1453.
[2]	Li Y,Fang F,Ma N,et al.Feasibility Study of Transthoracic Echocardiography for Coronary Slow Flow Phenomenon Evaluation:Validation by Coronary Angiography[J].Microcirculation,2016,23:277-282.
[3]	Ye MF,Zhao ZW,Luo YK,et al.Elevated endocan concentration is associated with coronary slow flow[J].Scand J Clin Lab Invest,2016,76:345-348.
[4]	Jin X,Chen Y,Chen H,et al.Evaluation of tumor-derived exosomal miRNA as potential diagnostic biomarkers for early stage non-small-cell lung cancer using next-generation sequencing[J].Clin Cancer Res,2017,23:5311-5319.
[5]	Li Y,Cai B,Shen L,et al.MiRNA-29b suppresses tumor growth through simultaneously inhibiting angiogenesis and tumorigenesis by targeting Akt3[J].Cancer Lett,2017,397:111-119.
[6]	Bastami M,Nariman-Saleh-Fam Z,Saadatian Z,et al.The miRNA Targetome of Coronary Artery Disease is Perturbed by Functional Polymorphisms Identified and Prioritized by in-depth Bioinformatics Analyses Exploiting Genome-wide Association Studies[J].Gene,2016,594:74-81.
[7]	Feldman A,Moreira DAR,Gun C,et al.Analysis of Circulating miR-1,miR-23a,and miR-26a in Atrial Fibrillation Patients Undergoing Coronary Bypass Artery Grafting Surgery[J].Ann Hum Genet,2017,81:99-105.
[8]	Hao L,Wang XG,Cheng JD,et al.The up-regulation of endothelin-1 and down-regulation of miRNA-125a-5p,-155,and-199a/b-3p in human atherosclerotic coronary artery[J].Cardiovasc Pathol,2014,23:217-223.
[9]	黄珊,李天发,杨洋,等.冠心病患者血清miRNA155与CRP水平变化及其相关性研究[J].医学临床研究,2015,32(12):2432-2434.
[10]	王磊,王红娜,祖晓麟.血浆miR-126水平与冠状动脉慢血流现象的关系[J].中华医学杂志,2019,99(17):1323-1327.
[11]	Akboga MK,Canpolat U,Balci KG,et al.Increased Platelet to Lymphocyte Ratio is Related to Slow Coronary Flow[J].Angiology.2016,67:21-26.
[12]	杨倩倩,崔建国,王树娟,等.冠状动脉慢血流患者的影响因素分析[J].中国循环杂志,2017,32(9):877-881.
[13]	Wang GK,Zhu JQ,Zhang JT,et al.Circulating microRNA:a novel potential biomarker for early diagnosis of acute myocardial infarction in humans[J].Eur Heart J,2010,31:659-666.
[14]	Faraoni I,Antonetti FR,Cardone J,et al.miR-155 gene:a typical multifunctional microRNA[J].Biochim Biophys Acta,2009,1792:497-505.
[15]	Zhang J,Zhao F,Yu X,et al.MicroRNA-155 modulates the proliferation of vascular smooth muscle cells by targeting endothelial nitric oxide synthase[J].Int J Mol Med,2015,35:1708-1714.
[16]	Yang Z,Zheng B,Zhang Y,et al.miR-155-dependent regulation of mammalian sterile 20-like kinase 2(MST2) coordinates inflammation,oxidative stress and proliferation in vascular smooth muscle cells[J].Biochim Biophys Acta,2015,1852:1477-1489.
[17]	余永芬.冠状动脉慢血流患者血液黏稠度变化分析[J].山西医科大学学报,2013,44(12):945-947.