Measurement of serum thrombopoietin levels and serum interlukin-11 levels in patients with thrombocytopenia with different causes and its significance
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摘要: 目的:探讨血小板减少症患者血清促血小板生成素(TPO)和白细胞介素-11(IL-11)水平及其与外周血小板计数之间的关系。方法:141例患者分为ITP组、血液肿瘤组和实体肿瘤组3组,应用双抗夹心ABC-ELISA法测定TPO、IL-11,同时用自动血细胞仪测定其血小板数,以30 例健康人为正常对照。结果:ITP组、血液肿瘤组和实体肿瘤组的血清TPO水平分别为(130.5±88.3)pg/ml、(103.5±83.9)pg/ml和(148.1±92.5)pg/ml,各组与对照组比较差异无统计学意义。ITP组、血液肿瘤组和实体肿瘤组的血清IL-11水平分别为(46.8±35.8)pg/ml、(40.3±24.7)pg/ml和(44.5±22.3)pg/ml;对照组则为(36.0±18.7)pg/ml。ITP组和实体肿瘤组血清IL-11水平明显高于对照组(均P<0.05)。结论:不同原因血小板减少症患者血清TPO、IL-11水平不一致,检测其有助于了解不同原因血小板减少的可能机制和指导临床治疗。Abstract: Objective:To investigate levels of endogenous thrombopoietin(TPO)and endogenous interlukin-11(IL-11),and the relationships among TPO,IL-11,and peripheral blood platelet counts in patients with thrombocytopenia. Method:One hundred and forty-one patients were classified into 3 groups including idiopathic thrombocytopenic purpura(ITP)group,hematological malignant patients group,and tumor group.Thirty healthy subjects were enrolled as controls.The levels of TPO and IL-11 were detected in 161 patients with throm bocytopenia and 30 healthy controls by double antibody sandwich enzyme linked immunoso rbent assay(ELISA),and the results were analyzed statistically. Result:The TPO levels in ITP group,hematological malignant patients group,tumor group and control group were (130.5±88.3)pg/ml,(103.5±83.9)pg/ml,(148.1±92.5)pg/ml and(138.5±53.9)pg/ml.Compared with the control group there were not statistically significant.The IL-11 levels in ITP group,hematological malignant patients group,tumor group and control group were(46.8±35.8)pg/ml,(40.3±24.7)pg/ml,(44.5±22.3)pg/ml,and (36.0±18.7)pg/ml.The IL-11 levels in ITP group and tumor group were higher compared to those in control group.Conclusion:The levels of serum TPO and IL-11 in thrombocytopenia patients with different causes are different.Detecting the levels of TPO and IL-11 in patients with thrombocytopenia will contribute to understand the mechanism of thrombocytopenia and direct its clinical therapy.
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Key words:
- thrombocytopenia /
- thrombopoietin /
- interlukin-11
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